[针灸对海洛因复吸大鼠条件性位置偏爱及前额叶皮质超微结构的影响]

[Influence of acupuncture and moxibustion on conditional position preference and prefrontal cortical ultrastructure in heroin re-addicted rats].

作者信息

Zhang Rong-Jun, Song Xiao-Ge, Cai Xing-Hui

机构信息

Department of Anatomy, Anhui College of Chinese Medicine, Hefei 230038, China.

出版信息

Zhen Ci Yan Jiu. 2009 Apr;34(2):97-100.

PMID:19685722

Abstract

OBJECTIVE

To observe the effect of acupuncture and moxibustion on the conditional position preference (CPP) and prefrontal cortical ultrastructure in heroin re-addicted rats.

METHODS

Thirty-two Wistar rats were randomly divided into normal, model, medication and acu-moxibustion (acu-moxi) groups, with 8 cases in each. Re-addiction model was established by repeated intramuscular injection of heroin into the hindlimbs. Rats of medication group were treated with intragastric administration of Methadone during detoxification. For rats in acu-moxi group, acupuncture needle was inserted into "Baihui" (GV 20) and moxibustion treatment was used to bilateral "Shenshu" (BL 23) for 30 min during detoxification. Rats in normal group were given with intramuscular injection of normal saline during addiction. CPP tests were conducted once daily in a spatial place preference box for 8 days. On the 39th day of the experiment, the rats under anesthesia were killed for sampling the prefrontal cortex (PFC) tissue on an ice plate, then, the ultrastructure of the neurons was observed by using a transmission electron microscope.

RESULTS

After modeling, the rats' staying duration in the dark-box was shortened obviously (P < 0. 1). After the treatment, the staying duration in dark-box in acu-moxi group was significantly longer than that in model and medication groups (P < 0.01). Compared with normal control group, electron-microscopic results indicated that in PFC tissues of model and medication groups, vacuoles and edema of cytoplasm at different degrees, enlargement of the rough endoplasmic reticulum (REPR), vacuoles or disappearance of mitochondria, reduction of ribosome in number, and widening of the nuclear intermembrance interstice were seen. While in acu-moxi group, the number of mitochondria and ribosome increased slightly, the mitochondrial cristae was clear, the number of REPR increased relatively but dilated slightly, and the intermembrance space was basically normal.

CONCLUSION

Acu-moxibustion can improve heroin re-addicted rats' conditional position preference and has a protection effect on the cerebral cortical neurons. disappearance of mitochondria, reduction of ribosome in number, and widening of the nuclear intermembrance interstice were seen. While in acu-moxi group, the number of mitochondria and ribosome increased slightly, the mitochondrial cristae was clear, the number of REPR increased relatively but dilated slightly, and the intermembrance space was basically normal.

CONCLUSION

Acu-moxibustion can improve heroin re-addicted rats' conditional position preference and has a protection effect on the cerebral cortical neurons.

摘要

目的

观察针灸对海洛因复吸大鼠条件性位置偏爱（CPP）及前额叶皮质超微结构的影响。

方法

将32只Wistar大鼠随机分为正常组、模型组、药物组和针灸组，每组8只。通过后肢重复肌肉注射海洛因建立复吸模型。药物组大鼠在戒毒期间采用美沙酮灌胃治疗。针灸组大鼠在戒毒期间针刺“百会”（GV 20）并艾灸双侧“肾俞”（BL 23）30分钟。正常组大鼠在成瘾期间肌肉注射生理盐水。在空间位置偏爱箱中每日进行1次CPP测试，共8天。实验第39天，将麻醉状态下的大鼠处死，在冰盘上取前额叶皮质（PFC）组织，然后用透射电子显微镜观察神经元的超微结构。

结果

造模后，大鼠在暗箱中的停留时间明显缩短（P < 0.1）。治疗后，针灸组大鼠在暗箱中的停留时间明显长于模型组和药物组（P < 0.01）。与正常对照组相比，电镜结果显示模型组和药物组PFC组织中出现不同程度的细胞质空泡化和水肿、粗面内质网（REPR）扩张、线粒体空泡化或消失、核糖体数量减少以及核膜间隙增宽。而针灸组线粒体和核糖体数量略有增加，线粒体嵴清晰，REPR数量相对增加但轻度扩张，膜间隙基本正常。