Tang Shuang-Hong, Du Yan-Jun, Tao Yi-Ming, Tian Qing, Kong Li-Hong
College of Acupuncture-Moxibustion and Orthopaedics, Hubei University of CM, Wuhan 430061, China.
School of Basic Medicine, Tongji Medical College, Huazhong University of Science and Technology.
Zhongguo Zhen Jiu. 2019 Mar 12;39(3):281-6. doi: 10.13703/j.0255-2930.2019.03.015.
To observe the effects of acupuncture at "Baihui" (GV 20) and "Shenshu" (BL 23) on the ultrastructure of hippocampal dentate gyrus in rats with Alzheimer's disease.
Forty SPF Wistar male rats were randomly divided into a normal group, a sham operation group, a model group and an acupuncture group, 10 rats in each one. The rats in the model group and the acupuncture group were treated with injection of 5 μL Aβ at bilateral hippocampus, while the rats in the sham operation group were treated with injection of 5 μL 0.9% NaCl. Three days after modeling, the rats in the acupuncture group were treated with acupuncture at "Baihui" (GV 20) and "Shenshu" (BL 23) for 20 min, once a day, six treatments constituted a course, and totally two courses were given with an interval of 1 day between courses. The rats in the other groups received normal diet and no treatment was given. Before modeling, four days after modeling and after treatment, water maze test was performed to observe the escape latency and the number of crossing platforms. The hippocampal dentate gyrus was collected and transmission electron microscope was applied to observe the ultrastructure changes of neurons and astrocytes.
①Four days after modeling, compared with the normal group and the sham operation group, the escape latency was significantly prolonged and the number of crossing platforms was reduced in the model group (all <0.01); after treatment, compared with the model group, the escape latency was significantly reduced and the number of crossing platforms was increased in the acupuncture group (both <0.01). ②In the normal group and the sham operation group, the morphology of neurons and astrocytes was intact, the nuclear and membrane structure were clear, and the morphology of organelles such as mitochondria, endoplasmic reticulum and lysosomes was normal. In the model group, the morphology of neurons was irregular, the nucleus was severely constricted with edema in the cytoplasm, the color of heterochromatin was deepened, the endoplasmic reticulum was expanded, the granulation was removed and the number of mitochondria was decreased, even with malformed-like change in mitochondrial cristae; there was severe edema around astrocytes, few organelles in the cytoplasm, severe swelling of mitochondria and mild expansion of the endoplasmic reticulum. In the acupuncture group, the edema of the neuron and astrocytes was still evident, and the mitochondrial was mildly swollen but relieved compared with that in the model group, and there were no obvious abnormalities in neuronal endoplasmic reticulum and ribosomes.
Acupuncture could improve the ultrastructure of neurons and astrocytes in the hippocampal dentate gyrus in rats with Alzheimer's disease induced by Aβ.
观察针刺“百会”（GV 20）、“肾俞”（BL 23）对阿尔茨海默病大鼠海马齿状回超微结构的影响。
将40只SPF级雄性Wistar大鼠随机分为正常组、假手术组、模型组和针刺组，每组10只。模型组和针刺组大鼠双侧海马注射5 μL Aβ，假手术组大鼠双侧海马注射5 μL 0.9%氯化钠溶液。造模3天后，针刺组大鼠针刺“百会”（GV 20）、“肾俞”（BL 23）20分钟，每日1次，6次为1个疗程，共进行2个疗程，疗程间隔1天。其他组大鼠正常饮食，不做处理。造模前、造模后4天及治疗后，进行水迷宫试验，观察逃避潜伏期及穿越平台次数。取海马齿状回，应用透射电子显微镜观察神经元及星形胶质细胞超微结构变化。
①造模后4天，与正常组和假手术组比较，模型组逃避潜伏期显著延长，穿越平台次数减少（均P<0.01）；治疗后，与模型组比较，针刺组逃避潜伏期显著缩短，穿越平台次数增加（均P<0.01）。②正常组和假手术组神经元及星形胶质细胞形态完整，核膜结构清晰，线粒体、内质网、溶酶体等细胞器形态正常。模型组神经元形态不规则，核严重皱缩，胞质水肿，异染色质颜色加深，内质网扩张，颗粒脱失，线粒体数量减少，线粒体嵴呈畸形样改变；星形胶质细胞周围严重水肿，胞质内细胞器少，线粒体严重肿胀，内质网轻度扩张。针刺组神经元及星形胶质细胞水肿仍较明显，线粒体轻度肿胀，但较模型组减轻，神经元内质网及核糖体无明显异常。
针刺可改善Aβ诱导的阿尔茨海默病大鼠海马齿状回神经元及星形胶质细胞超微结构。
