[一种中药方剂对模拟失重状态下大鼠股骨钙沉积的影响：采用（41）Ca示踪-加速器质谱分析]

[Effect of a Chinese herbal prescription on femur calcium deposition in rats under simulated weightlessness: by using (41)Ca tracing-accelerator mass spectrometry analysis].

作者信息

Hu Sumin, Zhou Peng, Jiang Shan, He Ming, Fu Qian, Yang Jiajia, Gao Xuemin

机构信息

School of Preclinical Medicine, Beijing University of Chinese Medicine, Beijing 100029, China.

出版信息

Zhongguo Zhong Yao Za Zhi. 2009 May;34(9):1129-32.

PMID:19685751

Abstract

UNLABELLED

To study the effect of a Chinese herbal prescription on external calcium deposition to weight-bearing bone in simulated weightlessness rats.

METHOD

Twenty-one male Wistar rats were divided into 3 groups: control group, tail suspension group, tail suspension with Chinese medicine group which takes a Chinese herbal prescription extract (containing Radix Rehmanniae Preparata, Radix Acanthopanacis Bidentatae, Radix Astragali, Radix Angelicae Sinensis, Concha Ostreae prepared by acetic acid) by intragastric administration. After 1 week adaption, there start off 3 weeks simulated weightlessness by tail suspension. At the eleventh day of simulated weightlessness, every rat was given one equal dose of 41Ca tracer by intragastric administration. Right femurs were separated as experiment over, and the ratio of 41Ca to 40Ca (41Ca/40Ca) was measured by accelerator mass spectrometry (AMS), while total femur calcium was measured by inductively coupled plasma-atomic emission spectroscopy (ICP-AES). Femur 41Ca deposition amount (DA) and femur 41Ca deposition ratio (DR) were calculated.

RESULT

The results showed that compared with control group, 41Ca/40Ca decreased significantly (P < 0.001) in tail suspension group, while in tail suspension with Chinese medicine group, it significantly increased (P < 0.05). DA and DR were both decreased significantly (P < 0.001) in tail suspension group, but no significant change in tail suspension with Chinese medicine group as compared with control group. Compared with tail suspension group, DA and DR increased significantly (P < 0.001) in tail suspension with Chinese medicine group.

CONCLUSION

Simulated weightlessness by tail suspension can cause decreased deposition of external calcium to weight-bearing bone, and the Chinese herbal prescription in this trial is effective to prevent the decrease. Moreover, multiple mechanisms may contribute to weightlessness induced osteoporosis, besides calcium deposition disturbance.

摘要

未标注

研究一种中药复方对模拟失重大鼠负重骨外源性钙沉积的影响。

方法

将21只雄性Wistar大鼠分为3组：对照组、尾部悬吊组、中药尾部悬吊组，中药尾部悬吊组灌胃给予一种中药复方提取物（含熟地黄、二齿五加、黄芪、当归、醋制牡蛎）。适应1周后，通过尾部悬吊开始3周的模拟失重。在模拟失重的第11天，每只大鼠灌胃给予等量的41Ca示踪剂。实验结束后分离右侧股骨，用加速器质谱法（AMS）测定41Ca与40Ca的比值（41Ca/40Ca），同时用电感耦合等离子体原子发射光谱法（ICP-AES）测定股骨总钙含量。计算股骨41Ca沉积量（DA）和股骨41Ca沉积率（DR）。