National Food Research Institute, Tsukuba, Ibaraki, Japan.
FEMS Microbiol Lett. 2009 Oct;299(1):95-9. doi: 10.1111/j.1574-6968.2009.01733.x. Epub 2009 Jul 22.
A vanillin-tolerant strain of Saccharomyces cerevisiae was screened and its intracellular ergosterol levels were compared with several laboratory yeast strains to study the potential relationship between ergosterol content and vanillin tolerance. Saccharomyces cerevisiae NBRC1950 was selected as a vanillin-tolerant strain. Its ergosterol content was higher than those of the laboratory strains. The results of DNA microarray and quantitative reverse transcriptase-polymerase chain reaction analysis showed that five genes involved in ergosterol biosynthesis (ERG28, HMG1, MCR1, ERG5, and ERG7) were upregulated in NBRC 1950 compared with strain X2180, suggesting that high expression of genes involved in ergosterol biosynthesis may cause high ergosterol content in strain NBRC 1950. The S. cerevisiae HX strain, which was a high-ergosterol-containing strain derived from X2180, was more tolerant to vanillin than the parental strain, suggesting that high ergosterol content may, in part, be responsible for vanillin tolerance. These findings provide a biotechnological basis for the molecular engineering of S. cerevisiae with increased tolerance to vanillin.
筛选出了一株耐香草醛的酿酒酵母菌株，并将其细胞内麦角固醇水平与几种实验室酵母菌株进行比较，以研究麦角固醇含量与香草醛耐受性之间的潜在关系。选择酿酒酵母 NBRC1950 作为耐香草醛菌株，其麦角固醇含量高于实验室菌株。DNA 微阵列和定量逆转录聚合酶链反应分析结果表明，与 X2180 菌株相比，NBRC1950 中参与麦角固醇生物合成的五个基因（ERG28、HMG1、MCR1、ERG5 和 ERG7）上调，表明参与麦角固醇生物合成的基因高表达可能导致 NBRC1950 菌株中麦角固醇含量高。从 X2180 衍生的高麦角固醇含量的 S. cerevisiae HX 菌株比亲本菌株对香草醛更耐受，表明高麦角固醇含量可能部分导致了香草醛耐受性。这些发现为提高酿酒酵母对香草醛耐受性的分子工程提供了生物技术基础。
