Department of General Surgery, Gulhane Military Medical Faculty, Ankara, Turkey.
J Gastroenterol Hepatol. 2009 Nov;24(11):1763-7. doi: 10.1111/j.1440-1746.2009.05907.x. Epub 2009 Aug 3.
Intra-abdominal adhesions are important postoperative complications following abdominal surgery. The adhesions that develop form the basis of more advanced pathology such as intestinal obstruction or infertility. Melatonin is secreted from the pineal gland in a circadian pattern; this molecule has potent antioxidant characteristics and has beneficial effects in many models of inflammation. The aim of this study was to evaluate the effects of melatonin on peritoneal adhesions created in rats.
A total of 28 Sprague-Dawley male rats were used and divided into four groups. In the first phase of the study, pinealectomy (PINX) was performed on half the animals. An incision was made and sutured in the cecum of all experimental animals in all groups 15 days after the PINX procedure. Some animals were given melatonin orally at a dose of 5 mg/kg daily following the adhesion operation and continued for 15 days. The rats were anesthetized and the abdomen opened after the 15th day (on day 30 of the study). After adhesion scoring based on macroscopic inspection, tissue samples were obtained from the sutured region of the cecum to measure malondialdehyde and hydroxyproline.
Peritoneal adhesion density was significantly higher in the PINX group compared to the control animals; exogenously administered melatonin significantly reduced adhesion formation. The degree of adhesion was also significantly lower in the intact rats given melatonin compared to the control group.
Antioxidant activity increases in the oxidative process. We conclude that melatonin may be an important molecule in preventing peritoneal adhesions.
腹部手术后，腹腔粘连是一种重要的术后并发症。形成的粘连是更严重的病理情况的基础，如肠梗阻或不孕。褪黑素由松果腺按照昼夜节律分泌；这种分子具有很强的抗氧化特性，并对许多炎症模型具有有益的作用。本研究旨在评估褪黑素对大鼠腹膜粘连形成的影响。
共使用 28 只雄性 Sprague-Dawley 大鼠，分为四组。在研究的第一阶段，对一半动物进行松果腺切除术（PINX）。所有实验组动物在 PINX 手术后 15 天，均在盲肠处做切口并缝合。一些动物在粘连手术后每天给予 5mg/kg 的褪黑素口服，并持续 15 天。第 15 天（研究的第 30 天），大鼠麻醉后打开腹部。根据宏观检查进行粘连评分后，从盲肠缝合区获取组织样本，以测量丙二醛和羟脯氨酸。
与对照组动物相比，PINX 组的腹膜粘连密度明显更高；外源性给予褪黑素可显著减少粘连形成。与对照组相比，给予褪黑素的完整大鼠的粘连程度也明显较低。
氧化过程中抗氧化活性增加。我们得出结论，褪黑素可能是预防腹膜粘连的重要分子。
