Sabri Mohammed, Jeon Hyojin, Ai Jinglu, Tariq Asma, Shang Xueyuan, Chen Gang, Macdonald R Loch
Division of Neurosurgery, St. Michael's Hospital, Keenan Research Centre, Li Ka Shing Knowledge Institute of St. Michael's Hospital, Toronto, Ontario, Canada.
Brain Res. 2009 Oct 27;1295:179-85. doi: 10.1016/j.brainres.2009.08.021. Epub 2009 Aug 15.
A model of subarachnoid hemorrhage (SAH) first described in rats where blood is injected into the prechiasmatic cistern was adapted to mice. The hypothesis was that such an anterior circulation SAH model would produce vasospasm of greater severity and longer duration than other mouse models. The goal was to create a mouse model that could then be used in transgenic and knockout animals in order to further knowledge of SAH and vasospasm. A needle was inserted stereotactically into the prechiasmatic cistern and 100 microl autologous arterial blood injected over seconds (n=10). Effects were compared to injection of saline (n=10) or to sham operation (n=7). Monitoring of cerebral blood flow by laser Doppler showed a statistically similar decrease during injection in both groups. 7 days after SAH there was vasospasm of the middle and anterior cerebral arteries (51% reduction in MCA radius in SAH compared to saline-injected group, P<0.009, Student's t-test). In order to determine if SAH in this model was associated with neuronal injury, brains were examined for TUNEL and fluoro-jade-positive cells. 60% of SAH but not saline-injected mice exhibited TUNEL-positive cells in the cerebral cortex and 30% of the SAH but no saline-injected mice had fluoro-jade positive cells in the cortex, hippocampus and dentate gyrus. The model is simple to perform and may be useful for investigating the pathophysiology of SAH.
一种最初在大鼠中描述的蛛网膜下腔出血（SAH）模型，即将血液注入视交叉前池，已被应用于小鼠。其假设是，这种前循环SAH模型会比其他小鼠模型产生更严重、持续时间更长的血管痉挛。目标是创建一种小鼠模型，然后可用于转基因和基因敲除动物，以进一步了解SAH和血管痉挛。通过立体定向将一根针插入视交叉前池，并在数秒内注入100微升自体动脉血（n = 10）。将结果与注入生理盐水（n = 10）或假手术（n = 7）进行比较。激光多普勒监测脑血流显示，两组在注射过程中脑血流下降在统计学上相似。SAH后7天，大脑中动脉和前动脉出现血管痉挛（与注入生理盐水组相比，SAH组大脑中动脉半径减少51%，P<0.009，Student t检验）。为了确定该模型中的SAH是否与神经元损伤有关，对大脑进行了TUNEL和氟玉髓阳性细胞检查。60%的SAH小鼠而非注入生理盐水的小鼠在大脑皮层中表现出TUNEL阳性细胞，30%的SAH小鼠而非注入生理盐水的小鼠在皮层、海马体和齿状回中有氟玉髓阳性细胞。该模型操作简单，可能有助于研究SAH的病理生理学。
