Zhang Rui, Khan Dilaware, Muhammad Sajjad
Department of Neurosurgery, Medical Faculty, University Hospital Düsseldorf, Heinrich- Heine-Universität, Mooren Str. 5, 40225 , Düsseldorf, Germany.
Department of Neurology, First Medical Center of PLA General Hospital, PLA Medical College, Beijing, China.
Sci Rep. 2025 Apr 21;15(1):13769. doi: 10.1038/s41598-025-97603-1.
The Circle of Willis perforation (cWp) mouse model is widely used in subarachnoid hemorrhage (SAH) research but involves sacrificing the external carotid artery (ECA), which may have a potential affect on the hemodynamic in the carotid arteries and cortical perfusion. We propose a modified approach using needle puncture via the common carotid artery (CCA) to preserve carotid vascular integrity. Twenty-seven C57BL/6 mice were randomly assigned into three groups and underwent cWp surgery in three procedures: sham (n = 3), ECA (n = 12), and CCA (n = 12). Surgical duration, success rate, intraoperative intracranial pressure (ICP) fluctuations, 24-hour mortality, and neurological deficits were assessed. The CCA approach achieved a 100% success rate and shorter surgical duration than the ECA approach (ECA 73 ± 18 vs. CCA 36 ± 10 min, P < 0.05). ICP fluctuations and mortality rates were comparable between the ECA and CCA groups (P > 0.05), indicating that the CCA approach shared a similar pattern with the ECA approach. Neurological outcomes were similar across SAH groups (CCA SAH induction and ECA SAH induction) but worse than sham (P < 0.05) in terms of body weight loss, open-filed test and Rotarod test performances. This modified cWp CCA approach, which preserves the carotid structures, helps eliminate hemodynamic bias and offers a potentially more efficient alternative with a shorter surgical duration compared to the classical ECA approach. It may prove to be a valuable option for broader application in SAH preclinical research.
Willis环穿孔（cWp）小鼠模型广泛应用于蛛网膜下腔出血（SAH）研究，但该模型需要牺牲颈外动脉（ECA），这可能会对颈动脉血流动力学和皮质灌注产生潜在影响。我们提出一种改良方法，即通过颈总动脉（CCA）进行针刺，以保留颈动脉血管的完整性。将27只C57BL/6小鼠随机分为三组，分三个步骤进行cWp手术：假手术组（n = 3）、ECA组（n = 12）和CCA组（n = 12）。评估手术持续时间、成功率、术中颅内压（ICP）波动、24小时死亡率和神经功能缺损情况。与ECA方法相比，CCA方法成功率达到100%，且手术持续时间更短（ECA组73±18分钟，CCA组36±10分钟，P < 0.05）。ECA组和CCA组之间的ICP波动和死亡率相当（P > 0.05），表明CCA方法与ECA方法具有相似的模式。在SAH组（CCA SAH诱导组和ECA SAH诱导组）中，神经功能结局相似，但在体重减轻、旷场试验和转棒试验表现方面比假手术组差（P < 0.05）。这种改良的cWp CCA方法保留了颈动脉结构，有助于消除血流动力学偏差，与经典的ECA方法相比，手术持续时间更短，可能是一种更有效的替代方法。它可能被证明是在SAH临床前研究中更广泛应用的一个有价值的选择。
