Koekemoer Andrea L, Chong Nelson W, Goodall Alison H, Samani Nilesh J
Department of Cardiovascular Sciences, University of Leicester, Glenfield General Hospital, Leicester, UK.
FEBS Lett. 2009 Sep 3;583(17):2964-7. doi: 10.1016/j.febslet.2009.08.011. Epub 2009 Aug 15.
Myocyte stress 1 (MS1) is a recently described striated muscle actin-binding protein that is up-regulated in the early stages of pressure overload left ventricular hypertrophy. The aim of this study was to determine whether MS1 induces cellular hypertrophy and protects against apoptosis. Over-expressed MS1 co-localized with actin in H9c2 cells and altered expression of genes of the myocardin-related transcription factor (MRTF)/serum response factor (SRF) transcriptional pathways and in addition the apoptosis repressor with caspase recruitment domain (Nol3) gene. The size of cells over-expressing MS1 was significantly increased by 55% and over-expression of MS1 dramatically inhibited staurosporine-induced apoptosis by 89%. These findings suggest the involvement of MS1 in cellular hypertrophy and protection against apoptosis.
肌细胞应激蛋白1（MS1）是一种最近被描述的横纹肌肌动蛋白结合蛋白，在压力超负荷左心室肥大的早期阶段上调。本研究的目的是确定MS1是否诱导细胞肥大并防止细胞凋亡。过表达的MS1在H9c2细胞中与肌动蛋白共定位，并改变了心肌相关转录因子（MRTF）/血清反应因子（SRF）转录途径以及含半胱天冬酶募集结构域的凋亡抑制因子（Nol3）基因的表达。过表达MS1的细胞大小显著增加了55%，并且MS1的过表达显著抑制了星形孢菌素诱导的细胞凋亡，抑制率达89%。这些发现表明MS1参与细胞肥大和抗细胞凋亡过程。
