Zhang Ke, Fang Huafeng, Shen Gang, Taylor John-Stephen A, Wooley Karen L
Washington University, Department of Chemistry, One Brookings Drive, CB1134, Saint Louis, MO 63130, USA.
Proc Am Thorac Soc. 2009 Aug 15;6(5):450-7. doi: 10.1513/pats.200902-010AW.
This mini-review highlights developments that have been made over the past year to advance the construction of well-defined nanoscale objects to serve as devices for cell transfection. Design of the nanoscale objects originated from biomimicry concepts, using histones as the model, to afford cationic shell crosslinked knedel-like (cSCK) nanoparticles. Packaging and delivery of plasmid DNA, oligonucleotides, and peptide nucleic acids were studied by dynamic light scattering, transmission electron microscopy, gel electrophoresis, biological activity assays, RT-PCR measurements, flow cytometry, and confocal fluorescence microscopy. With the demonstration of more efficient cell transfection in vitro than that achieved using commercially-available transfection agents, together with the other features offered by the robust nanostructural framework, work continues toward the application of these cSCKs for in vivo molecular recognition of genetic material, for imaging and therapy targeted specifically to pulmonary injury and disease.
本综述重点介绍了过去一年在推进构建定义明确的纳米级物体作为细胞转染装置方面所取得的进展。纳米级物体的设计源自仿生学概念，以组蛋白为模型，制备出阳离子壳交联类克内德尔（cSCK）纳米颗粒。通过动态光散射、透射电子显微镜、凝胶电泳、生物活性测定、逆转录-聚合酶链反应测量、流式细胞术和共聚焦荧光显微镜研究了质粒DNA、寡核苷酸和肽核酸的包装与递送。由于已证明这些纳米颗粒在体外比市售转染试剂具有更高的细胞转染效率，再加上其坚固的纳米结构框架所具备的其他特性，目前仍在继续致力于将这些cSCK应用于体内遗传物质的分子识别，以及针对肺损伤和疾病的特异性成像与治疗。
