与细胞穿透肽（HIV Tat PTD）偶联的纳米颗粒对CHO细胞摄取的形状效应。
Shape effects of nanoparticles conjugated with cell-penetrating peptides (HIV Tat PTD) on CHO cell uptake.
作者信息
Zhang Ke, Fang Huafeng, Chen Zhiyun, Taylor John-Stephen A, Wooley Karen L
机构信息
Department of Chemistry, Washington University School of Arts and Sciences, 1 Brookings Drive, Saint Louis, Missouri 63130, USA.
出版信息
Bioconjug Chem. 2008 Sep;19(9):1880-7. doi: 10.1021/bc800160b. Epub 2008 Aug 9.
Abstract
In order to probe the nanoparticle shape/size effect on cellular uptake, a spherical and two cylindrical nanoparticles, whose lengths were distinctively varied, were constructed by the selective cross-linking of amphiphilic block copolymer micelles. Herein, we demonstrate that, when the nanoparticles were functionalized with the protein transduction domain of human immunodeficiency virus type 1 Tat protein (HIV Tat PTD), the smaller, spherical nanoparticles had a higher rate of cell entry into Chinese hamster ovary (CHO) cells than did the larger, cylindrical nanoparticles. It was also found that nanoparticles were released after internalization and that the rate of cell exit was dependent on both the nanoparticle shape and the amount of surface-bound PTD.
摘要
为了探究纳米颗粒形状/尺寸对细胞摄取的影响，通过两亲性嵌段共聚物胶束的选择性交联构建了一种球形和两种圆柱形纳米颗粒，其长度有明显差异。在此，我们证明，当纳米颗粒用1型人类免疫缺陷病毒Tat蛋白（HIV Tat PTD）的蛋白转导结构域功能化时，较小的球形纳米颗粒比更大的圆柱形纳米颗粒进入中国仓鼠卵巢（CHO）细胞的速率更高。还发现纳米颗粒在内化后会释放，并且细胞排出速率取决于纳米颗粒形状和表面结合的PTD量。
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