Soliman Mohamed Mohamed, El-Senosi Yakut Abdel-Fattah, Salem Maysara Mahmoud, Abdel Hamid Omniya Mahmoud, Kazuhiro Kimura
Department of Biochemistry, Faculty of Veterinary Medicine, Benha University, 020-013, Egypt.
J Vet Sci. 2009 Sep;10(3):197-201. doi: 10.4142/jvs.2009.10.3.197.
Treatment of AIDS (HIV) and hepatitis C virus needs protease inhibitors (PI) to prevent viral replication. Uses of PI in therapy are usually associated with a decrease in body weight and dyslipidemia. Acylation stimulating protein (ASP) is a protein synthesized in adipocytes to increase triglycerides biosynthesis, for that the relation of PI and ASP to adipogenesis is tested in this work. ASP expression was increased during 3T3-L1 differentiation and reached a peak at day 8 with cell maturation. Addition of PI during adipocytes differentiation dose dependently and significantly (p < 0.5) inhibited the degree of triglycerides (TG) accumulation. Moreover, presence of ASP (450 ng/mL) in media significantly (p < 0.5) stimulated the degree of TG accumulation and there was additive stimulation for ASP when added with insulin (10 microg/mL). Finally, when ASP in different doses (Low, 16.7; Medium, 45 and High, 450 ng/mL) incubated with a dose of x150 PI, ASP partially inhibited the PI-inhibited adipogenesis and TG accumulation. The results in this study show that PI inhibit lipids accumulation and confirm role of ASP in TG biosynthesis and adipogenesis.
艾滋病（HIV）和丙型肝炎病毒的治疗需要蛋白酶抑制剂（PI）来阻止病毒复制。PI在治疗中的使用通常与体重减轻和血脂异常有关。酰化刺激蛋白（ASP）是一种在脂肪细胞中合成的蛋白质，用于增加甘油三酯的生物合成，因此在本研究中测试了PI和ASP与脂肪生成的关系。在3T3-L1分化过程中，ASP表达增加，并在第8天随着细胞成熟达到峰值。在脂肪细胞分化过程中添加PI呈剂量依赖性，且显著（p < 0.5）抑制甘油三酯（TG）的积累程度。此外，培养基中存在ASP（450 ng/mL）显著（p < 0.5）刺激了TG的积累程度，并且当与胰岛素（10 μg/mL）一起添加时，对ASP有累加刺激作用。最后，当不同剂量（低剂量，16.7；中剂量，45；高剂量，450 ng/mL）的ASP与150倍剂量的PI一起孵育时，ASP部分抑制了PI抑制的脂肪生成和TG积累。本研究结果表明，PI抑制脂质积累，并证实了ASP在TG生物合成和脂肪生成中的作用。
