van der Pal R H, Klein W, van Golde L M, Lopes-Cardozo M
Laboratory of Veterinary Biochemistry, Utrecht University, The Netherlands.
Biochim Biophys Acta. 1990 Mar 12;1043(1):91-6. doi: 10.1016/0005-2760(90)90114-d.
The in vivo metabolism of sulfatides was studied in spinal cord and cerebral cortex of developing rat pups. Developmental changes in the rate of sulfolipid synthesis were measured after the intraperitoneal injection of 35SO4(2-). We also measured the accumulation of sulfatides, as well as the profiles of cerebroside sulfotransferase, cerebroside sulfatase and arylsulfatase A in both brain regions as a function of postnatal development. The accumulation of sulfatides was higher in spinal cord than in cerebral cortex. In addition, sulfatide metabolism was more active in spinal cord. In both brain regions, the developmental pattern of 35SO4(2-) incorporation into sulfolipids was closely correlated to the activities of cerebroside sulfotransferase and of arylsulfatase A. The activity of these enzymes was initially low, increased during the period of active myelination and declined thereafter. However, the activity of cerebroside sulfatase, measured with its physiological substrate, [35S]sulfatide, increased during development and did not decline. An explanation for the difference between the developmental profiles of the arylsulfatase A and cerebroside sulfatase reactions (which are supposed to be catalysed by the same enzyme) is proposed.
在发育中的幼鼠脊髓和大脑皮层中研究了硫脂的体内代谢。腹腔注射35SO4(2-)后，测定了硫脂合成速率的发育变化。我们还测量了两个脑区中硫脂的积累，以及脑苷脂硫酸转移酶、脑苷脂硫酸酯酶和芳基硫酸酯酶A的分布情况，作为出生后发育的函数。脊髓中硫脂的积累高于大脑皮层。此外，脊髓中硫脂代谢更活跃。在两个脑区中，35SO4(2-)掺入硫脂的发育模式与脑苷脂硫酸转移酶和芳基硫酸酯酶A的活性密切相关。这些酶的活性最初较低，在活跃髓鞘形成期增加，此后下降。然而，用其生理底物[35S]硫脂测量的脑苷脂硫酸酯酶的活性在发育过程中增加且未下降。提出了对芳基硫酸酯酶A和脑苷脂硫酸酯酶反应（假定由同一种酶催化）发育概况差异的解释。
