Rao Raghu Ambekar Ramachandra, Mehta Monal R, Toussaint Kimani C
Photonics Research of Bio/nano Environments (PROBE), Department of Electrical and Computer Engineering, University of Illinois Urbana-Champaign, 1406 W Green St, Urbana, IL 61801, USA.
Opt Express. 2009 Aug 17;17(17):14534-42. doi: 10.1364/oe.17.014534.
Fourier transform-second-harmonic generation imaging is employed to obtain quantitative metrics of collagen fibers in biological tissues. In particular, the preferred orientation and maximum spatial frequency of collagen fibers for selected regions of interest in porcine trachea, ear, and cornea are determined. These metrics remain consistent when applied to collagen fibers in the ear, which can be expected from observation. Collagen fibers in the trachea are more random with large standard deviations in orientation, and large variations in maximum spatial frequency. In addition, these metrics are used to investigate structural changes through a 3D stack of the cornea. This technique can be used as a quantitative marker to assess the structure of collagen fibers that may change due to damage from disease or physical injury.
傅里叶变换二次谐波产生成像用于获取生物组织中胶原纤维的定量指标。具体而言，确定了猪气管、耳朵和角膜中选定感兴趣区域的胶原纤维的优选取向和最大空间频率。当应用于耳朵中的胶原纤维时，这些指标保持一致，这从观察中是可以预期的。气管中的胶原纤维更加随机，取向的标准偏差较大，最大空间频率变化也较大。此外，这些指标用于通过角膜的三维堆栈研究结构变化。该技术可作为一种定量标记物，用于评估可能因疾病或物理损伤而受损的胶原纤维结构。
