Kang DongKyun, Yelin Dvir, Bouma Brett E, Tearney Guillermo J
Harvard Medical School and Wellman center for Photomedicine, Massachusetts General Hospital, 55 Fruit Street, Boston, MA 02114, USA.
Opt Express. 2009 Aug 17;17(17):15239-47. doi: 10.1364/oe.17.015239.
Spectrally-encoded endoscopy (SEE) is a technique for ultraminiature endoscopy that encodes each spatial location on the sample with a different wavelength. One limitation of previous incarnations of SEE is that it inherently creates monochromatic images, since the spectral bandwidth is expended in the spatial encoding process. Here we present a spectrally-encoded imaging system that has color imaging capability. The new imaging system utilizes three distinct red, green, and blue spectral bands that are configured to illuminate the grating at different incident angles. By careful selection of the incident angles, the three spectral bands can be made to overlap on the sample. To demonstrate the method, a bench-top system was built, comprising a 2400-lpmm grating illuminated by three 525-microm-diameter beams with three different spectral bands. Each spectral band had a bandwidth of 75 nm, producing 189 resolvable points. A resolution target, color phantoms, and excised swine small intestine were imaged to validate the system's performance. The color SEE system showed qualitatively and quantitatively similar color imaging performance to that of a conventional digital camera.
光谱编码内窥镜检查（SEE）是一种超微型内窥镜检查技术，它用不同波长对样本上的每个空间位置进行编码。SEE先前版本的一个局限性在于，由于光谱带宽在空间编码过程中被消耗，它本质上会生成单色图像。在此，我们展示了一种具有彩色成像能力的光谱编码成像系统。新的成像系统利用三个不同的红、绿、蓝光谱带，这些光谱带被配置为以不同入射角照射光栅。通过仔细选择入射角，可使这三个光谱带在样本上重叠。为演示该方法，构建了一个台式系统，该系统包括一个由三束直径为525微米、具有三个不同光谱带的光束照射的2400线/毫米光栅。每个光谱带的带宽为75纳米，可产生189个可分辨点。对分辨率测试图、彩色模型和切除的猪小肠进行成像，以验证该系统的性能。彩色SEE系统在定性和定量方面均显示出与传统数码相机相似的彩色成像性能。
