Mirfakhrai M, Tanaka Y, Yanagisawa K
Institute of Biological Sciences, University of Tsukuba, Ibaraki, Japan.
Genetics. 1990 Mar;124(3):607-13. doi: 10.1093/genetics/124.3.607.
Restriction fragment length polymorphisms (RFLPs) were used as markers to monitor mitochondrial inheritance in the cellular slime mold, Polysphondylium pallidum. When two opposite mating types (mat1 and mat2) of closely related strains were crossed, all the haploid progeny regardless of mating type inherited their mitochondrial DNA from the mat2 parent only. When opposite mating types from more distantly related strains were crossed, most of the progeny also inherited their mitochondrial DNA from the mat2 parent, but some inherited their mitochondrial DNA from the mat1 parent. In both cases however, the transmission of mitochondrial DNA was uniparental, since in every individual progeny only one type of mitochondrial DNA exists. Moreover, in crosses involving more distantly related strains all the progeny of a single macrocyst were shown to contain the same type of mitochondrial DNA. These findings are discussed in regard to mechanisms of transmission and the possible involvement of nuclear genes in the control of transmission of mitochondrial DNA in Polysphondylium.
限制性片段长度多态性（RFLPs）被用作标记，以监测细胞黏菌苍白聚孢霉中的线粒体遗传。当两个亲缘关系密切的菌株的两种相反交配型（mat1和mat2）杂交时，所有单倍体后代无论交配型如何，都仅从mat2亲本继承其线粒体DNA。当亲缘关系较远的菌株的相反交配型杂交时，大多数后代也从mat2亲本继承其线粒体DNA，但有些从mat1亲本继承其线粒体DNA。然而，在这两种情况下，线粒体DNA的传递都是单亲的，因为在每个个体后代中只存在一种类型的线粒体DNA。此外，在涉及亲缘关系较远的菌株的杂交中，单个大孢囊的所有后代都显示含有相同类型的线粒体DNA。本文就苍白聚孢霉中线粒体DNA的传递机制以及核基因在控制线粒体DNA传递中可能的作用对这些发现进行了讨论。
