Xu Jian, Liu Xiaoli, Yang Xuefeng, Guo Huailan
Shenzhen Center for Chronic Disease Prevention and Control, Shenzhen 518020, China.
Wei Sheng Yan Jiu. 2009 Jul;38(4):398-400.
To study the mechanism of the disorder of thyroid hormone metabolism resulted from iodine excess in order to seek suitable selenium intervention dosage.
140 Balb/c mice were randomly divided into seven groups, the normal control group, the excessive iodine group (drank the water containing potassium iodate 3000 microg/L) and five selenium intervention groups (drank the water containing 3000 microg/L of potassium iodate and 0.1, 0.2, 0.3, 0.4 and 0.5 mg/L of selenium). All of the mice were cultivated for 16 weeks and the thyroid hormone in plasma were assayed by radioimmunoassay. The iodine in urine and thyroid were analyzed by Cer-Arsenite colormetric method. The activities of glutathione peroxidase, superoxide dismutase and thyroid peroxidase and the level of malondialdehyde in thyroid were analyzed.
The level of thyroid hormone of selenium intervention groups had no significant difference with that of normal control group (P > 0.05). Compared with excessive iodine group, the iodine in thyroid of 0.2 mg/L selenium intervention group increased significantly (P < 0.05). Compared with the normal control group, the activities of glutathione peroxidase and superoxide dismutase and the level of malondialdehyde of 0.2-0.3 mg/L selenium intervention groups in thyroid were not significantly different. Compared with the normal control group, the activity of thyroid peroxidase of 0.1-0.3 mg/L selenium intervention groups were not significantly different.
The results indicated that the optimal dose of selenium could restrain the disorder of thyroid hormone metabolism induced by excessive iodine in mice.
研究碘过量致甲状腺激素代谢紊乱的机制，以寻求合适的硒干预剂量。
将140只Balb/c小鼠随机分为7组，即正常对照组、碘过量组（饮用含碘酸钾3000μg/L的水）和5个硒干预组（饮用含3000μg/L碘酸钾及0.1、0.2、0.3、0.4和0.5mg/L硒的水）。所有小鼠饲养16周，采用放射免疫法测定血浆甲状腺激素水平。用亚砷酸铈比色法分析尿碘和甲状腺碘含量。分析甲状腺中谷胱甘肽过氧化物酶、超氧化物歧化酶和甲状腺过氧化物酶的活性以及丙二醛水平。
硒干预组甲状腺激素水平与正常对照组相比差异无统计学意义（P>0.05）。与碘过量组相比，0.2mg/L硒干预组甲状腺碘含量显著升高（P<0.05）。与正常对照组相比，0.2 - 0.3mg/L硒干预组甲状腺中谷胱甘肽过氧化物酶和超氧化物歧化酶活性及丙二醛水平差异无统计学意义。与正常对照组相比，0.1 - 0.3mg/L硒干预组甲状腺过氧化物酶活性差异无统计学意义。
结果表明，适宜剂量的硒可抑制小鼠碘过量所致的甲状腺激素代谢紊乱。
