Mikołajczyk Tomasz P, Skrzeczyńska-Moncznik Joanna E, Zarebski Mirosław A, Marewicz Ewa A, Wiśniewska Anna M, Dzieba Magdalena, Dobrucki Jerzy W, Pryjma Juliusz R
Department of Immunology, Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University, Gronostajowa, Cracow, Poland.
Immunology. 2009 Sep;128(1):103-13. doi: 10.1111/j.1365-2567.2009.03087.x.
Macrophages have the potential to recognize apoptotic neutrophils and phagocytose them while the same function for monocytes is uncertain. In fact, early findings indicated that monocytes started to phagocytose neutrophils on the third day of differentiation to macrophages. Here we show, using flow cytometry and confocal microscopy, that peripheral blood monocytes phagocytose apoptotic but not freshly isolated granulocytes. Recognition of apoptotic cells is predominantly connected with CD16(+) monocytes (CD14(high) CD16(+) and CD14(dim) CD16(+)) and requires CD36. Clearance of apoptotic polymorphonuclear leucocytes appears to be independent of the CD14 mechanism. Uptake of apoptotic Jurkat T cells by monocytes is CD14 and CD36 dependent. Liposomes containing phosphatidyl-l-serine reduce binding of apoptotic polymorphonuclear leucocytes. Lipopolysaccharide-activated subpopulations of monocytes while in contact with apoptotic cells produce more anti-inflammatory cytokine interleukin-10 whereas the production of pro-inflammatory cytokines, tumour necrosis factor-alpha and interleukin-1beta is reduced.
巨噬细胞有识别凋亡中性粒细胞并将其吞噬的潜力，而单核细胞是否具有相同功能尚不确定。事实上，早期研究结果表明，单核细胞在分化为巨噬细胞的第三天开始吞噬中性粒细胞。在此，我们通过流式细胞术和共聚焦显微镜显示，外周血单核细胞可吞噬凋亡的粒细胞，但不能吞噬刚分离的粒细胞。对凋亡细胞的识别主要与CD16(+)单核细胞（CD14(高)CD16(+)和CD14(低)CD16(+)）有关，且需要CD36。凋亡多形核白细胞的清除似乎与CD14机制无关。单核细胞对凋亡Jurkat T细胞的摄取是CD14和CD36依赖性的。含有磷脂酰丝氨酸的脂质体可减少凋亡多形核白细胞的结合。脂多糖激活的单核细胞亚群在与凋亡细胞接触时会产生更多的抗炎细胞因子白细胞介素-10，而促炎细胞因子肿瘤坏死因子-α和白细胞介素-1β的产生则减少。
