KLOTHO基因多态性G-395A和C1818T与日本血液透析患者的低密度脂蛋白胆固醇和尿酸有关。

KLOTHO gene polymorphisms G-395A and C1818T are associated with low-density lipoprotein cholesterol and uric acid in Japanese hemodialysis patients.

作者信息

Shimoyama Yasuhiko, Taki Kentaro, Mitsuda Yoko, Tsuruta Yoshinari, Hamajima Nobuyuki, Niwa Toshimitsu

机构信息

Department of Advanced Medicine for Uremia, Nagoya University School of Medicine, Nagoya, Japan.

出版信息

Am J Nephrol. 2009;30(4):383-8. doi: 10.1159/000235686. Epub 2009 Aug 19.

DOI:10.1159/000235686

PMID:19690404

Abstract

BACKGROUND/AIMS: This study aimed to investigate the association of the KLOTHO gene single nucleotide polymorphisms (SNPs), G-395A and C1818T, with various laboratory data in 219 Japanese hemodialysis (HD) patients.

METHODS

The genotyping of G-395A in the promoter region and C1818T in exon 4 was performed using polymerase chain reaction with confronting two-pair primers (PCR-CTPP) assay.

RESULTS

In HD patients, the allele frequencies of G-395A were 0.847 for the G allele and 0.153 for the A allele and those of the C1818T were 0.829 for the C allele and 0.171 for the T allele. There were no significant differences in allele frequencies of G-395A and those of the C1818T between HD patients and healthy subjects. Multivariate analysis adjusted for age and duration on HD demonstrated that uric acid was significantly high in A allele carriers of G-395A compared with GG genotype in all and female patients. Low-density lipoprotein cholesterol was significantly low in T allele carriers of C1818T compared with CC genotype in all and male patients.

CONCLUSION

KLOTHO gene SNPs G-395A and C1818T are associated with low-density lipoprotein cholesterol and uric acid in HD patients.

摘要

背景/目的：本研究旨在调查219名日本血液透析（HD）患者中，KLOTHO基因单核苷酸多态性（SNP），即G-395A和C1818T，与各种实验室数据之间的关联。

方法

采用两对引物对抗聚合酶链反应（PCR-CTPP）法对启动子区域的G-395A和外显子4中的C1818T进行基因分型。

结果

在HD患者中，G-395A的等位基因频率为：G等位基因为0.847，A等位基因为0.153；C1818T的等位基因频率为：C等位基因为0.829，T等位基因为0.171。HD患者与健康受试者之间，G-395A和C1818T的等位基因频率无显著差异。对年龄和HD病程进行校正的多变量分析表明，在所有患者及女性患者中，G-395A的A等位基因携带者的尿酸显著高于GG基因型。在所有患者及男性患者中，C1818T的T等位基因携带者的低密度脂蛋白胆固醇显著低于CC基因型。