Department of Experimental Physics, University of Pécs, Ifjúság u. 6., Pécs, H-7624, Hungary.
J Fluoresc. 2010 Jan;20(1):87-94. doi: 10.1007/s10895-009-0526-3. Epub 2009 Aug 19.
Determination of concentrations of spectrally overlapping compounds has special difficulties. Several methods are available to calculate the constituents' concentrations in moderately complex mixtures. A method which can provide information about spectrally hidden components in mixtures is very useful. Two methods powerful in resolving spectral components are compared in this paper. The first method tested is the Derivative Matrix Isopotential Synchronous Fluorimetry (DMISF). It is based on derivative analysis of MISF spectra, which are constructed using isopotential trajectories in the Excitation-Emission Matrix (EEM) of background solution. For DMISF method, a mathematical routine fitting the 3D data of EEMs was developed. The other method tested uses classical Least Squares Fitting (LSF) algorithm, wherein Rayleigh- and Raman-scattering bands may lead to complications. Both methods give excellent sensitivity and have advantages against each other. Detection limits of DMISF and LSF have been determined at very different concentration and noise levels.
测定光谱重叠化合物的浓度有特殊的困难。有几种方法可用于计算中等复杂混合物中各成分的浓度。一种能够提供混合物中光谱隐藏成分信息的方法是非常有用的。本文比较了两种在解析光谱成分方面非常有效的方法。第一种测试方法是导数矩阵等势能同步荧光法（DMISF）。它基于 MISF 光谱的导数分析，该光谱是使用背景溶液的激发-发射矩阵（EEM）中的等势能轨迹构建的。对于 DMISF 方法，开发了一个用于拟合 EEM 的 3D 数据的数学例程。另一种测试方法使用经典的最小二乘拟合（LSF）算法，其中瑞利和拉曼散射带可能会导致复杂情况。这两种方法都具有出色的灵敏度，并且彼此各有优势。DMISF 和 LSF 的检测限在非常不同的浓度和噪声水平下确定。
