Centre for Genome Research, Department of Microbiology and Biotechnology Centre, Faculty of Science, The M S. University of Baroda, Vadodara, Gujarat, 390002, India.
Transgenic Res. 2010 Jun;19(3):373-84. doi: 10.1007/s11248-009-9315-7. Epub 2009 Aug 19.
Transgenic rice (Oryza sativa L. cv. Pusa basmati 1), overexpressing the Rs-AFP2 defensin gene from the Raphanus sativus was generated by Agrobacterium tumefaciens-mediated transformation. Expression levels of Rs-AFP2 ranged from 0.45 to 0.53% of total soluble protein in transgenic plants. It was observed that constitutive expression of Rs-AFP2 suppresses the growth of Magnaporthe oryzae and Rhizoctonia solani by 77 and 45%, respectively. No effect on plant morphology was observed in the Rs-AFP2 expressing rice lines. The inhibitory activity of protein extracts prepared from leaves of Rs-AFP2 plants on the in vitro growth of M. oryzae indicated that the Rs-AFP2 protein produced by transgenic rice plants was biologically active. Transgene expression of Rs-AFP2 was not accompanied by an induction of pathogenesis-related (PR) gene expression, suggesting that the expression of Rs-AFP2 directly inhibits the pathogens. Here, we demonstrate that transgenic rice plants expressing the Rs-AFP2 gene show enhanced resistance to M. oryzae and R. solani, two of the most important pathogens of rice.
转基因为 Rs-AFP2 防御素的水稻（Oryza sativa L. cv. Pusa basmati 1）通过根瘤农杆菌介导的转化生成，该防御素来自萝卜（Raphanus sativus）。Rs-AFP2 在转基因植物中的表达水平范围为总可溶性蛋白的 0.45%至 0.53%。观察到 Rs-AFP2 的组成型表达分别抑制稻瘟病菌和立枯丝核菌的生长 77%和 45%。在表达 Rs-AFP2 的水稻品系中未观察到对植物形态的影响。从 Rs-AFP2 植株叶片制备的蛋白提取物对稻瘟病菌体外生长的抑制活性表明，转基水稻植株产生的 Rs-AFP2 蛋白具有生物活性。Rs-AFP2 的转基因表达不伴随病程相关（PR）基因表达的诱导，这表明 Rs-AFP2 的表达直接抑制了病原体。在这里，我们证明表达 Rs-AFP2 基因的转基因水稻植株对稻瘟病菌和立枯丝核菌这两种水稻最重要的病原体表现出增强的抗性。
