Tarakhovsky A M, Resnikov M, Zaichuk T, Tugusheva M V, Butenko Z A, Prassolov V S
Department of Leukemiagenesis, Academy Sciences UkrSSR, Kiev.
Oncogene. 1990 Mar;5(3):405-10.
The NEU proto-oncogene encodes a 185,000 dalton transmembrane glycoprotein with extensive homology to epidermal growth factor receptor. In the current study the effect of exogenous NEU expression on phenotype and growth properties of cells established lines was examined. The replication defective retroviruses were used to express constitutively NEU cDNA in the Rat-1, NIH3T3 and Balb/c3T3 cells. In spite of the practically similar NEU mRNA and protein content in infected cells only in Balb/c3T3 cells, high NEU expression ultimately led to oncogenic transformation. The Rat-1 cells were practically insensitive to oncogenic action of NEU. Subpopulation divergency with respect to NEU-dependent transformation was also revealed in infected NIH3T3 cells. These results suggest the existence of unknown host-specific factor(s) determining the response of cells to NEU overexpression.
NEU原癌基因编码一种185,000道尔顿的跨膜糖蛋白，与表皮生长因子受体具有广泛的同源性。在当前研究中，检测了外源性NEU表达对细胞系表型和生长特性的影响。复制缺陷型逆转录病毒用于在大鼠-1、NIH3T3和Balb/c3T3细胞中组成性表达NEU cDNA。尽管感染细胞中NEU mRNA和蛋白质含量实际上相似，但仅在Balb/c3T3细胞中，高NEU表达最终导致致癌转化。大鼠-1细胞对NEU的致癌作用实际上不敏感。在感染的NIH3T3细胞中也发现了与NEU依赖性转化相关的亚群差异。这些结果表明存在未知的宿主特异性因子，决定细胞对NEU过表达的反应。
