Corvinus University of Budapest, Budapest, Hungary.
Foodborne Pathog Dis. 2009 Nov;6(9):1133-40. doi: 10.1089/fpd.2009.0286.
Listeria monocytogenes is a foodborne pathogen capable of employing stress adaptive responses to evade a variety of stressors including temperature stress. We employed two-dimensional gel electrophoresis coupled with matrix-assisted laser desorption/ionization-time of flight analysis to study the differential expression of L. monocytogenes (ATCC 43256) soluble proteins at heat shock (60 degrees C) conditions, prolonged heat shock (60 degrees C for 9 minutes) conditions, and thermotolerance-inducing (48 degrees C for 30 minutes followed by 60 degrees C for 9 minutes) conditions. We compared the proteome of L. monocytogenes under these conditions to the proteome at 37 degrees C. Eighteen proteins were differentially expressed at 60 degrees C (6 up-regulated and 12 down-regulated), 21 proteins were differentially expressed (12 up-regulated and 9 down-regulated) when the cells were exposed to 60 degrees C for 9 minutes, and 20 proteins were differentially expressed (10 up-regulated and 10 down-regulated) when cells were initially exposed to 48 degrees C for 30 minutes before 60 degrees C for 9 minutes. There was one unidentifiable protein with observed molecular weight of 50 kDa which was differentially expressed across the three temperature treatments. Thermotolerance-inducing conditions caused the up-regulation of a protein by as much as 12-fold. DnaN, a previously identified stress protein, was up-regulated almost threefold at 60 degrees C. TcsA, a lipoprotein (CD4 T cell-stimulating antigen), and Gap (glyceraldehyde-3-phosphate-dehydrogenase) were selectively expressed under prolonged heat shock conditions suggesting their potential as candidate marker proteins targets for identifying temperature-stressed L. monocytogenes cells.
李斯特菌是一种食源性病原体，能够通过适应应激反应来逃避多种应激源，包括温度应激。我们采用二维凝胶电泳结合基质辅助激光解吸/电离飞行时间分析技术，研究了李斯特菌（ATCC 43256）在热激（60°C）条件下、延长热激（60°C 持续 9 分钟）条件下和耐热诱导（48°C 持续 30 分钟后 60°C 持续 9 分钟）条件下可溶性蛋白的差异表达。我们将这些条件下李斯特菌的蛋白质组与 37°C 下的蛋白质组进行了比较。在 60°C 时，有 18 种蛋白差异表达（6 种上调，12 种下调），在 60°C 持续 9 分钟时，有 21 种蛋白差异表达（12 种上调，9 种下调），在初始暴露于 48°C 持续 30 分钟后再暴露于 60°C 持续 9 分钟时，有 20 种蛋白差异表达（10 种上调，10 种下调）。在三种温度处理中，有一个未识别的蛋白分子量为 50 kDa，表现出差异表达。耐热诱导条件导致一种蛋白的上调幅度高达 12 倍。DnaN，一种先前鉴定的应激蛋白，在 60°C 时上调近三倍。TcsA，一种脂蛋白（CD4 T 细胞刺激抗原）和 Gap（甘油醛-3-磷酸脱氢酶）在延长热激条件下选择性表达，表明它们可能是识别处于温度应激状态的李斯特菌细胞的候选标记蛋白靶标。
