N-乙酰半胱氨酸对 (12)C(6+)离子照射诱导的小鼠淋巴细胞 DNA 损伤及免疫功能改变的影响。

Effect of N-acetylcysteine on (12)C(6+) ion irradiation-induced lymphocytes DNA damages and immunity changes in mice.

机构信息

Research Center for Heavy Ion Radiation Biology and Medicine, Institute of Modern Physics, Chinese Academy of Science, Lanzhou, China.

出版信息

J Radiat Res. 2009 Nov;50(6):567-71. doi: 10.1269/jrr.09047. Epub 2009 Aug 21.

DOI:10.1269/jrr.09047

PMID:19696471

Abstract

The aims of present study are to estimate the biological risks to the immunity of mice exposed to heavy ion radiation and to investigate the effect of N-acetylcysteine (NAC) on (12)C(6+) ion irradiation-induced lymphocyte DNA damage. Results showed that in the brine group, the levels of lymphocyte DNA damage and MN, thymocytes G(2)/M phase arrest and apoptosis percentages (except for activity of NK cells) were up at each time point. A time-response curve for MN and DNA damage appeared in the NAC group. We found that whole-body (12)C(6+) ion irradiation at a dose of 4 Gy could: induce lymphocyte DNA double-strand breaks (DSBs); immunocytes DSBs may lead to acute effects on immunity; and 200 mg/kg NAC showed significant protection against radiation harm.

摘要

本研究旨在评估小鼠暴露于重离子辐射后的免疫生物学风险，并探讨 N-乙酰半胱氨酸（NAC）对（12)C(6+)离子照射诱导的淋巴细胞 DNA 损伤的影响。结果表明，在盐水组中，淋巴细胞 DNA 损伤和 MN、胸腺细胞 G(2)/M 期阻滞以及细胞凋亡百分比（NK 细胞活性除外）在各个时间点均升高。NAC 组中出现了 MN 和 DNA 损伤的时间反应曲线。我们发现，全身（12)C(6+)离子照射 4 Gy 可导致：淋巴细胞 DNA 双链断裂（DSBs）；免疫细胞的 DSBs 可能导致急性免疫效应；200mg/kg NAC 对辐射损伤具有显著的保护作用。