Boland L M, Dingledine R
Curriculum in Neurobiology, University of North Carolina, School of Medicine, Chapel Hill 27599-7365.
Brain Res Dev Brain Res. 1990 Feb 1;51(2):259-66. doi: 10.1016/0165-3806(90)90284-6.
The F-11 cell line is a fusion product of embryonic rat dorsal root ganglion (DRG) cells with mouse neuroblastoma cell line N18TG-2 (Platika, D., Boulos, M.H., Baizer, L. and Fishman, M.C., Proc. Natl. Acad. Sci. U.S.A., 82 (1985) 3499-3503). F-11 cells were uniformly labelled using a monoclonal antibody (RT-97) to the 200 kDa subunit of neurofilament protein, which labels a subpopulation of adult rat DRG neurons. F-11 cells did not stain for antigenic markers of fibroblasts or Schwann/satellite cells which are also present in DRG. Monoclonal antibodies that recognize cell surface carbohydrates have been shown to label subpopulations of DRG neurons. The stage-specific embryonic antigens SSEA-3 and SSEA-4, and the antigen recognized by B23D8, were expressed by some F-11 cells but not by the neuroblastoma parent of the hybrid cells. SSEA-3 was expressed by about 20% of the F-11 cells, whereas 40-60% expressed SSEA-4 or the antigen recognized by B23D8. The stability of F-11 cell subpopulations for sensory antigen expression was demonstrated by isolating single cells and growing the progeny as clonal lines. In some subclones, nearly 100% of the cells stably expressed SSEA-4 and/or B23D8, or failed to stain with anti-SSEA-4, anti-SSEA-3, or B23D8 over 12 passages. Other subclones were unstable for the expression of these antigens. This study demonstrates the derivation of the F-11 cell line from sensory neurons but also indicates that multiple phenotypes of varying stability are present in this line. This information is important for the use of this line as a model for DRG neurons.
F-11细胞系是胚胎大鼠背根神经节（DRG）细胞与小鼠神经母细胞瘤细胞系N18TG-2的融合产物（普拉蒂卡，D.，布罗斯，M.H.，拜泽尔，L.和菲什曼，M.C.，《美国国家科学院院刊》，82（1985）3499 - 3503）。使用针对神经丝蛋白200 kDa亚基的单克隆抗体（RT - 97）对F-11细胞进行均匀标记，该抗体可标记成年大鼠DRG神经元的一个亚群。F-11细胞未被DRG中也存在的成纤维细胞或雪旺氏/卫星细胞的抗原标志物染色。已证明识别细胞表面碳水化合物的单克隆抗体可标记DRG神经元的亚群。一些F-11细胞表达阶段特异性胚胎抗原SSEA - 3和SSEA - 4，以及被B23D8识别的抗原，但杂交细胞的神经母细胞瘤亲本不表达。约20%的F-11细胞表达SSEA - 3，而40 - 60%的细胞表达SSEA - 4或被B23D8识别的抗原。通过分离单个细胞并将后代培养为克隆系，证明了F-11细胞亚群在感觉抗原表达方面的稳定性。在一些亚克隆中，近100%的细胞在12代以上稳定表达SSEA - 4和/或B23D8，或不被抗SSEA - 4、抗SSEA - 3或B23D8染色。其他亚克隆在这些抗原的表达上不稳定。这项研究证明了F-11细胞系源自感觉神经元，但也表明该细胞系中存在多种稳定性不同的表型。这些信息对于将该细胞系用作DRG神经元模型非常重要。
