Lawson S N, Harper A A, Harper E I, Garson J A, Anderton B H
J Comp Neurol. 1984 Sep 10;228(2):263-72. doi: 10.1002/cne.902280211.
A monoclonal antibody (RT97) against neurofilament protein specifically and exclusively labelled a subpopulation of rat dorsal root ganglion (DRG) neurones. For seven ganglia (L4 and T13) studied quantitatively the frequency distribution histograms of the size of labelled cells could be fitted by a single normal distribution whose parameters were extremely close to those of the normally distributed large light cell population in that ganglion. On this basis and on the basis of a statistical analysis of the results it was suggested that this antibody can be used as a much needed specific label for the large light population of neurones in rat DRGs. The small dark neurone population was not labelled by this antibody. In one ganglion the subjective analysis of whether each neurone was labelled or not was directly compared with microdensitometric measurements of reaction product intensity. This analysis supported the above conclusion, and furthermore no subdivisions of the labelled population were apparent on the basis of neuronal size plotted against intensity of the reaction product. Other neuronal cell bodies strongly labelled by this antibody were found in association with small unlabelled neurones not only in DRGs, but also in the trigeminal ganglion, the vagal ganglia, and the mesencephalic V nucleus, all of which are made up of primary afferent neurones and all of which are completely or partially derived from the neural crest. Sympathetic and central nervous system neuronal cell bodies were unlabelled or occasionally very lightly labelled although immunoreactive fibres abound in the central nervous system.
一种抗神经丝蛋白的单克隆抗体（RT97）特异性且专一性地标记了大鼠背根神经节（DRG）神经元的一个亚群。对于定量研究的7个神经节（L4和T13），标记细胞大小的频率分布直方图可以用单一正态分布拟合，其参数与该神经节中呈正态分布的大的亮细胞群体的参数极为接近。基于此以及对结果的统计分析，有人提出该抗体可作为大鼠背根神经节中大量亮神经元群体急需的特异性标记物。小的暗神经元群体未被该抗体标记。在一个神经节中，对每个神经元是否被标记的主观分析与反应产物强度的显微密度测量直接进行了比较。该分析支持了上述结论，而且根据反应产物强度绘制的神经元大小，标记群体中没有明显的细分。不仅在背根神经节中，而且在三叉神经节、迷走神经节和中脑V核中，都发现与未标记的小神经元相关联的其他被该抗体强烈标记的神经元细胞体，所有这些神经节均由初级传入神经元组成，并且全部或部分来源于神经嵴。交感神经系统和中枢神经系统的神经元细胞体未被标记，或偶尔被非常轻微地标记，尽管中枢神经系统中有大量免疫反应性纤维。
