Yu Yang, Feng Qian, Zhang Ting, Ma Chun-Ya, Zhang Xiao-Juan, Ge Guo-Feng, Lin Zi-Lin, Pan Ji-Chun, Wang De-Qing, Luo Qun, Tian Ya-Ping
Department of Blood Transfusion, Center for Clinical Transfusion Medicine, PLA General Hospital, Beijing 100853, China.
Zhongguo Shi Yan Xue Ye Xue Za Zhi. 2009 Aug;17(4):1067-70.
This study was aimed to investigate the effect of leukocyte depletion by filtration on the quality of apheresis platelets. 20 units of donor apheresis platelets were randomly selected and were preserved with agitation at 20 - 24 degrees C for 24 - 96 hours, then were filtered on polyester flatbed filters. The platelet concentration, mean platelet volume (MPV), volume of apheresis platelets, leukocyte count, pH value, lactate dehydrogenase (LDH) concentration, K(+) concentration and CD62p expression level on surface of platelet membrane, were detected before and after filtration, as well as the rate of leukocyte depletion and platelet loss were calculated. The results showed that the leukocyte count after filtration was remarkably lower than that before filtration (p < 0.001), and the rate of leukocyte depletion was 99.97%. Platelet loss was approximately 8%, and obviously lower than that of the national standard (p < 0.001). MPV, pH value, K(+) and LDH concentration were not significantly different before and after filtration. Compared with platelets before filtration, CD62p expression level after filtration slightly decreased (p > 0.05). CD62p expression on surface of platelet membrane in perfusion fluid obtained from filter plate was obviously higher than that before filtration (p < 0.05). MA of platelet after filtration slightly decreased (p > 0.05). It is concluded that leukocyte and partial activated platelets can be removed efficiently by using polyester flatbed filters, and platelet loss is very low. Filtration does not adversely affect coagulation activity of the platelets in vitro. Apheresis platelets after filtration can fulfil quality requirements to prevent infection of cytomegalovirus and HLA alloimmunization.
本研究旨在探讨过滤去除白细胞对单采血小板质量的影响。随机选取20单位供者单采血小板，在20 - 24℃下振荡保存24 - 96小时，然后通过聚酯平板滤器进行过滤。检测过滤前后的血小板浓度、平均血小板体积（MPV）、单采血小板体积、白细胞计数、pH值、乳酸脱氢酶（LDH）浓度、K⁺浓度以及血小板膜表面CD62p表达水平，并计算白细胞去除率和血小板丢失率。结果显示，过滤后的白细胞计数显著低于过滤前（p < 0.001），白细胞去除率为99.97%。血小板丢失约为8%，明显低于国家标准（p < 0.001）。过滤前后MPV、pH值、K⁺和LDH浓度无显著差异。与过滤前的血小板相比，过滤后的CD62p表达水平略有下降（p > 0.05）。滤板流出液中血小板膜表面的CD62p表达明显高于过滤前（p < 0.05）。过滤后血小板的MA略有下降（p > 0.05）。结论是，使用聚酯平板滤器可有效去除白细胞和部分活化血小板，且血小板丢失率很低。过滤对体外血小板的凝血活性无不良影响。过滤后的单采血小板可满足预防巨细胞病毒感染和HLA同种免疫的质量要求。
