Department of Medicine and Epidemiology, School of Veterinary Medicine, University of California, Davis, CA 95616, USA.
Vet J. 2010 Oct;186(1):110-2. doi: 10.1016/j.tvjl.2009.07.008. Epub 2009 Aug 20.
The humoral immune response and fecal shedding of Lawsonia intracellularis was investigated in 20 weanling foals following intra-rectal administration of frozen-thawed or lyophilized avirulent live L. intracellularis vaccine. Foals received either 30 mL frozen-thawed or lyophilized vaccine intra-rectally, given twice, 4 weeks apart. Serum samples from each foal were collected every 4 weeks for 16 weeks following the first vaccination and tested for anti-L. intracellularis specific IgG by immunoperoxidase monolayer assay. Rectal swabs were collected every other day following the first vaccination for 4 weeks for detection of L. intracellularis by real-time PCR. Both vaccine formulations administered intra-rectally in weanling foals were safe and led to similar onset and duration of fecal shedding and measurable serum IgG response against L. intracellularis.
研究了在直肠内给予冷冻-解冻或冻干无毒活 Lawsonia intracellularis 疫苗后，20 头断奶驹的体液免疫反应和 Lawsonia intracellularis 的粪便脱落情况。每头驹经直肠内接受 30 毫升冷冻-解冻或冻干疫苗，两次给药，间隔 4 周。首剂接种后 16 周内，每 4 周采集每头驹的血清样本，通过免疫过氧化物酶单层测定法检测抗 Lawsonia intracellularis 特异性 IgG。首剂接种后 4 周内，每隔一天采集直肠拭子，通过实时 PCR 检测 Lawsonia intracellularis。在断奶驹中经直肠内给予这两种疫苗制剂均安全，且导致相似的粪便脱落开始时间和持续时间，以及针对 Lawsonia intracellularis 的可测量血清 IgG 反应。
