School of Pharmaceutical Science and Technology, Tianjin University, Weijin Road, Tianjin, China.
Steroids. 2009 Nov-Dec;74(13-14):1051-6. doi: 10.1016/j.steroids.2009.08.004. Epub 2009 Aug 20.
Rhizoma Paridis saponins (RPS) have been found to show strong antitumor activity. However, few studies have yet investigated its role on pulmonary metastasis treated with this herb. To investigate the molecular mechanisms related to metastasis, we studied RPS-treated T739 mice using histopathology, immunohistochemistry and reverse transcription polymerase chain reaction. Apoptosis was measured by TUNEL assay. As a result, RPS inhibited tumor growth by inducing apoptosis and upregulated the expression of TIMP-2 and down-regulated the level of MMP-2 and MMP-9. In conclusion, RPS is a potent anticancer agent that elicits programmed cell death and inhibits metastases in murine lung adenocarcinoma in vivo.
重楼皂苷（RPS）已被发现具有很强的抗肿瘤活性。然而，目前很少有研究调查该草药对肺转移的作用。为了研究与转移相关的分子机制，我们使用组织病理学、免疫组织化学和逆转录聚合酶链反应研究了 RPS 处理的 T739 小鼠。通过 TUNEL 测定法测量细胞凋亡。结果表明，RPS 通过诱导细胞凋亡抑制肿瘤生长，并上调 TIMP-2 的表达，下调 MMP-2 和 MMP-9 的水平。总之，RPS 是一种有效的抗癌剂，可诱导细胞程序性死亡并抑制体内肺腺癌的转移。
