评价四甲基罗丹明和黑洞猝灭剂 1 标记探针以及五种商业扩增混合物在 TaqMan 实时 RT-PCR 检测呼吸道病原体中的应用。
Evaluation of tetramethylrhodamine and black hole quencher 1 labeled probes and five commercial amplification mixes in TaqMan real-time RT-PCR assays for respiratory pathogens.
机构信息
Centers for Disease Control and Prevention, Division of Bacterial Diseases, Respiratory Diseases Branch, Atlanta, GA 30333, USA.
出版信息
J Virol Methods. 2009 Dec;162(1-2):288-90. doi: 10.1016/j.jviromet.2009.08.004. Epub 2009 Aug 20.
Tetramethylrhodamine (TAMRA) and black hole quencher 1 (BHQ1) quenched probes and five one-step RT-PCR kits were evaluated in TaqMan real-time RT-PCR assays for detection of respiratory pathogens. The intra-assay variability of the BHQ1 probes were 1.2-2.8-fold lower than those of the TAMRA probes. All kits amplified the specific targets, but differed in their sensitivity by up to 3 orders of magnitude. The AgPath-ID kit provided the best overall performance for all assay targets.
四甲基罗丹明（TAMRA）和黑洞猝灭剂 1（BHQ1）猝灭探针以及五种一步法 RT-PCR 试剂盒在用于检测呼吸道病原体的 TaqMan 实时 RT-PCR 检测中进行了评估。BHQ1 探针的内试验变异性比 TAMRA 探针低 1.2-2.8 倍。所有试剂盒均扩增了特定的靶标，但灵敏度差异高达 3 个数量级。AgPath-ID 试剂盒为所有检测靶标提供了最佳的整体性能。
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