Bradbury R S, Champion A C, Reid D W
Microbiology Department, Royal Hobart Hospital, Cardio-Respiratory Research Group, Menzies Research Institute, School of Medicine, University of Tasmania, Hobart, Tasmania.
J Hosp Infect. 2009 Oct;73(2):151-6. doi: 10.1016/j.jhin.2009.05.021. Epub 2009 Aug 21.
A genotypically indistinguishable strain of Pseudomonas aeruginosa (Australian epidemic strain III: AES III) has previously been found in a proportion of adults with cystic fibrosis (CF) in Tasmania, Australia. The aim of this study was to identify a source of these infections within the major tertiary referral hospital for the State of Tasmania, and to determine if this strain could be isolated from settings other than the CF lung. A total of 120 isolates of P. aeruginosa were collected from clinical and environmental sources within the hospital and from environmental locations in the hospital vicinity. These isolates were genotyped by random amplification of polymorphic DNA (RAPD)-polymerase chain reaction (PCR) and antimicrobial susceptibility testing was performed using the Clinical and Laboratory Standards Institute method. Confirmation of similar genotypes identified by RAPD-PCR was performed using pulsed-field gel electrophoresis with restriction enzyme SpeI. AES III was not recovered from any source other than the respiratory secretions of CF patients. P. aeruginosa in the non-CF settings was found to be panmictic, and no cross-infection or acquisition of hospital environment strains by patients was observed.
此前在澳大利亚塔斯马尼亚州的部分成年囊性纤维化（CF）患者中发现了一种基因分型无法区分的铜绿假单胞菌菌株（澳大利亚流行菌株III：AES III）。本研究的目的是在塔斯马尼亚州的主要三级转诊医院内确定这些感染的来源，并确定该菌株是否能从CF肺部以外的环境中分离出来。总共从医院内的临床和环境来源以及医院附近的环境位置收集了120株铜绿假单胞菌分离株。这些分离株通过随机扩增多态性DNA（RAPD）-聚合酶链反应（PCR）进行基因分型，并使用临床和实验室标准协会的方法进行抗菌药物敏感性测试。使用限制性内切酶SpeI的脉冲场凝胶电泳对通过RAPD-PCR鉴定的相似基因型进行确认。除了CF患者的呼吸道分泌物外，未从任何其他来源分离出AES III。在非CF环境中的铜绿假单胞菌被发现是随机交配的，并且未观察到患者之间的交叉感染或从医院环境菌株中获得感染。
