Lho D S, Hong J K, Paek H K, Lee J A, Park J
Doping Control Center, Korea Institute of Science and Technology, Cheongryang, Seoul.
J Anal Toxicol. 1990 Mar-Apr;14(2):77-83. doi: 10.1093/jat/14.2.77.
An analytical procedure for determination of phenolalkylamines, narcotic analgesics, and beta-blockers in urine by gas chromatography/mass spectrometry (GC/MS) is described. The detection of phenolalkylamines, narcotic analgesics, and beta-blockers is based on acid hydrolysis, liquid-liquid extraction, and selective derivatization. For screening of phenolalkylamines the m/e 179 and 267 ions were monitored by GC/MS. With narcotic analgesics, the extracted ion corresponded to the molecular ion (M+) of the drug and two additional characteristic ions. Beta-blockers were analyzed as the selectively derivatized forms of the parent molecule and its metabolites by GC/MS with selected ion monitoring. The ions monitored for screening of beta-blockers containing an isopropylamine group were m/e 284 and 129 ions. The ion at m/e 86 was monitored to characterize the tert-butylamine group of beta-blockers.
描述了一种通过气相色谱/质谱法（GC/MS）测定尿液中苯乙胺类、麻醉性镇痛药和β受体阻滞剂的分析方法。苯乙胺类、麻醉性镇痛药和β受体阻滞剂的检测基于酸水解、液液萃取和选择性衍生化。对于苯乙胺类的筛查，通过GC/MS监测m/e 179和267离子。对于麻醉性镇痛药，提取的离子对应于药物的分子离子（M+）和另外两个特征离子。β受体阻滞剂通过GC/MS采用选择离子监测法分析母体分子及其代谢物的选择性衍生化形式。用于筛查含异丙胺基团的β受体阻滞剂的监测离子为m/e 284和129离子。监测m/e 86处的离子以表征β受体阻滞剂的叔丁胺基团。
