Jodal M, Hallbäck D A, Svanvik J, Lundgren O
Acta Physiol Scand. 1975 Dec;95(4):441-7. doi: 10.1111/j.1748-1716.1975.tb10072.x.
A new perfusion technique has been developed for the study of net water transport across the intestinal epithelium in vivo. The lumen of an isolated intestinal segment is steadily perfused with a solution of known composition in a closed perfusion system with a reservoir large enough to prevent recirculation. The intestinal segment may be enclosed in a plethysmorgraph. Changes in the perfused volume is recorded by a volume transducer coupled to the recirculating system via a T-tube. If no motility occurs, the changes of the perfusion volume reflects net water transport across the intestinal epithelium. A quantitative comparison of this technique with the convention polyethylene glycol method revealed no significant difference. The plethysmorgraphic method also makes it possible to quantify the net water absorption via lymph and blood.
已开发出一种新的灌注技术，用于研究体内肠道上皮细胞的净水分转运。在一个封闭的灌注系统中，用已知成分的溶液稳定灌注分离肠段的肠腔，该系统有一个足够大的储液器以防止再循环。肠段可置于体积描记器中。通过经T形管与再循环系统相连的体积传感器记录灌注体积的变化。如果没有蠕动发生，灌注体积的变化反映了肠道上皮细胞的净水分转运。该技术与传统聚乙二醇方法的定量比较显示无显著差异。体积描记法还能够对通过淋巴和血液的净水分吸收进行定量。
