通过转座子诱变鉴定鲁氏耶尔森菌中的鞭毛运动基因
Identification of flagellar motility genes in Yersinia ruckeri by transposon mutagenesis.
作者信息
Evenhuis Jason P, Lapatra Scott E, Verner-Jeffreys David W, Dalsgaard Inger, Welch Timothy J
机构信息
National Center for Cool and Cold Water Aquaculture, Agricultural Research Service, U.S. Department of Agriculture, Kearneysville, West Virginia 25430, USA.
出版信息
Appl Environ Microbiol. 2009 Oct;75(20):6630-3. doi: 10.1128/AEM.01415-09. Epub 2009 Aug 21.
Abstract
Here we demonstrate that flagellar secretion is required for production of secreted lipase activity in the fish pathogen Yersinia ruckeri and that neither of these activities is necessary for virulence in rainbow trout. Our results suggest a possible mechanism for the emergence of nonmotile biotype 2 Y. ruckeri through the mutational loss of flagellar secretion.
摘要
在此我们证明，鞭毛分泌对于鱼类病原菌鲁氏耶尔森菌分泌脂肪酶活性的产生是必需的，并且这两种活性对于虹鳟鱼的致病性都不是必需的。我们的结果提示了一种通过鞭毛分泌的突变性丧失而出现鲁氏耶尔森菌非运动生物型2的可能机制。
相似文献
1
Identification of flagellar motility genes in Yersinia ruckeri by transposon mutagenesis.通过转座子诱变鉴定鲁氏耶尔森菌中的鞭毛运动基因
Appl Environ Microbiol. 2009 Oct;75(20):6630-3. doi: 10.1128/AEM.01415-09. Epub 2009 Aug 21.
2
The flagellar master operon flhDC is a pleiotropic regulator involved in motility and virulence of the fish pathogen Yersinia ruckeri.鞭毛主操纵子flhDC是一种多效调节因子，参与鱼类病原菌鲁氏耶尔森菌的运动性和毒力。
J Appl Microbiol. 2017 Mar;122(3):578-588. doi: 10.1111/jam.13374. Epub 2017 Jan 30.
3
Mutations involved in the emergence of Yersinia ruckeri biotype 2 in France.法国导致鱼类鲁氏耶尔森菌生物型 2 出现的突变。
Transbound Emerg Dis. 2019 May;66(3):1387-1394. doi: 10.1111/tbed.13175. Epub 2019 Apr 9.
4
Flagellar regulation mediated by the Rcs pathway is required for virulence in the fish pathogen Yersinia ruckeri.Rcs 途径介导的鞭毛调节是鱼类病原体鲁氏耶尔森菌毒力所必需的。
Fish Shellfish Immunol. 2019 Aug;91:306-314. doi: 10.1016/j.fsi.2019.05.036. Epub 2019 May 20.
5
In vitro cytotoxicity and multiplex PCR detection of virulence factors of Yersinia ruckeri isolated from rainbow trout in North West Germany.德国西北部虹鳟鱼分离出的鲁氏耶尔森菌的体外细胞毒性及毒力因子多重PCR检测
Berl Munch Tierarztl Wochenschr. 2014 May-Jun;127(5-6):233-42.
6
Genome Sequence of the Fish Pathogen Yersinia ruckeri SC09 Provides Insights into Niche Adaptation and Pathogenic Mechanism.鱼类病原菌鲁氏耶尔森菌SC09的基因组序列为生态位适应和致病机制提供了见解。
Int J Mol Sci. 2016 Apr 14;17(4):557. doi: 10.3390/ijms17040557.
7
Yersinia ruckeri Isolates Recovered from Diseased Atlantic Salmon (Salmo salar) in Scotland Are More Diverse than Those from Rainbow Trout (Oncorhynchus mykiss) and Represent Distinct Subpopulations.从苏格兰患病大西洋鲑（Salmo salar）中分离出的鲁氏耶尔森菌菌株比从虹鳟（Oncorhynchus mykiss）中分离出的菌株更多样化，且代表不同的亚群。
Appl Environ Microbiol. 2016 Sep 16;82(19):5785-94. doi: 10.1128/AEM.01173-16. Print 2016 Oct 1.
8
The UvrY response regulator of the BarA-UvrY two-component system contributes to Yersinia ruckeri infection of rainbow trout (Oncorhynchus mykiss).巴氏杆菌 UvrY 双组分系统的 UvrY 应答调节子有助于虹鳟鱼（Oncorhynchus mykiss）感染鲁氏耶尔森氏菌。
Arch Microbiol. 2010 Jul;192(7):541-7. doi: 10.1007/s00203-010-0582-8. Epub 2010 May 18.
9
The yctCBA operon of Yersinia ruckeri, involved in in vivo citrate uptake, is not required for virulence.鲁氏耶尔森菌（Yersinia ruckeri）体内柠檬酸摄取相关的 yctCBA 操纵子对于毒力并非必需。
Appl Environ Microbiol. 2011 Feb;77(3):1107-10. doi: 10.1128/AEM.01808-10. Epub 2010 Dec 3.
10
Multilocus Variable-Number Tandem-Repeat Analysis of Yersinia ruckeri Confirms the Existence of Host Specificity, Geographic Endemism, and Anthropogenic Dissemination of Virulent Clones.多基因座可变数目串联重复分析确认了鲑鱼气单胞菌的宿主特异性、地理特有性和毒力克隆的人为传播
Appl Environ Microbiol. 2018 Aug 1;84(16). doi: 10.1128/AEM.00730-18. Print 2018 Aug 15.
引用本文的文献
1
Biotyping reveals loss of motility in two distinct Yersinia ruckeri lineages exclusive to Norwegian aquaculture.生物分型揭示了仅存在于挪威水产养殖中的两个独特的鲁氏耶尔森氏菌谱系丧失了运动性。
J Fish Dis. 2022 May;45(5):641-653. doi: 10.1111/jfd.13590. Epub 2022 Feb 18.
2
A TIR Domain-Containing Protein (STIR-2) Mediates Immune Evasion by Targeting the MyD88 Adaptor.一个 TIR 结构域蛋白（STIR-2）通过靶向 MyD88 衔接子介导免疫逃逸。
Int J Mol Sci. 2019 Sep 7;20(18):4409. doi: 10.3390/ijms20184409.
3
Proteome analysis reveals a role of rainbow trout lymphoid organs during Yersinia ruckeri infection process.蛋白质组分析揭示了虹鳟鱼淋巴器官在鳗弧菌感染过程中的作用。
Sci Rep. 2018 Sep 18;8(1):13998. doi: 10.1038/s41598-018-31982-6.
4
The Infection Process of : Reviewing the Pieces of the Jigsaw Puzzle.感染过程：拼拼图游戏。
Front Cell Infect Microbiol. 2018 Jun 26;8:218. doi: 10.3389/fcimb.2018.00218. eCollection 2018.
5
Multilocus Variable-Number Tandem-Repeat Analysis of Yersinia ruckeri Confirms the Existence of Host Specificity, Geographic Endemism, and Anthropogenic Dissemination of Virulent Clones.多基因座可变数目串联重复分析确认了鲑鱼气单胞菌的宿主特异性、地理特有性和毒力克隆的人为传播
Appl Environ Microbiol. 2018 Aug 1;84(16). doi: 10.1128/AEM.00730-18. Print 2018 Aug 15.
6
Global proteomic profiling of Yersinia ruckeri strains.鲁氏耶尔森菌菌株的全蛋白质组分析
Vet Res. 2017 Sep 20;48(1):55. doi: 10.1186/s13567-017-0460-3.
7
Shotgun proteomic analysis of Yersinia ruckeri strains under normal and iron-limited conditions.正常和铁限制条件下鲁氏耶尔森菌菌株的鸟枪法蛋白质组学分析
Vet Res. 2016 Oct 6;47(1):100. doi: 10.1186/s13567-016-0384-3.
8
Yersinia ruckeri, the causative agent of enteric redmouth disease in fish.鲁氏耶尔森菌，鱼类肠道红嘴病的病原体。
Vet Res. 2015 Sep 24;46(1):103. doi: 10.1186/s13567-015-0238-4.
9
Complete Genome Sequence of Yersinia ruckeri Strain CSF007-82, Etiologic Agent of Red Mouth Disease in Salmonid Fish.鲑科鱼类红嘴病病原体鲁氏耶尔森菌CSF007-82菌株的全基因组序列
Genome Announc. 2015 Jan 29;3(1):e01491-14. doi: 10.1128/genomeA.01491-14.
10
Independent emergence of Yersinia ruckeri biotype 2 in the United States and Europe.美国和欧洲的鲁氏耶尔森菌生物型 2 独立出现。
Appl Environ Microbiol. 2011 May;77(10):3493-9. doi: 10.1128/AEM.02997-10. Epub 2011 Mar 25.
本文引用的文献
1
Yersinia ruckeri biotype 2 isolates from mainland Europe and the UK likely represent different clonal groups.来自欧洲大陆和英国的鲁氏耶尔森氏菌生物型2分离株可能代表不同的克隆群。
Dis Aquat Organ. 2009 Mar 9;84(1):25-33. doi: 10.3354/dao02039.
2
Coordinating assembly of a bacterial macromolecular machine.细菌大分子机器的协同组装
Nat Rev Microbiol. 2008 Jun;6(6):455-65. doi: 10.1038/nrmicro1887.
3
First report of Yersinia ruckeri biotype 2 in the USA.美国鲁氏耶尔森菌生物型2的首次报告。
J Aquat Anim Health. 2007 Mar;19(1):35-40. doi: 10.1577/H06-011.1.
4
sigma28-dependent transcription in Salmonella enterica is independent of flagellar shearing.肠炎沙门氏菌中依赖σ28的转录与鞭毛剪切无关。
J Bacteriol. 2006 Jul;188(14):5196-203. doi: 10.1128/JB.00299-06.
5
First description of non-motile Yersinia ruckeri serovar I strains causing disease in rainbow trout, Oncorhynchus mykiss (Walbaum), cultured in Spain.首次描述在西班牙养殖的虹鳟鱼（Oncorhynchus mykiss，Walbaum）中引起疾病的无运动性鲁氏耶尔森菌血清型I菌株。
J Fish Dis. 2006 Jun;29(6):339-46. doi: 10.1111/j.1365-2761.2006.00723.x.
6
Construction of a virulent, green fluorescent protein-tagged Yersinia ruckeri and detection in trout tissues after intraperitoneal and immersion challenge.构建一种带有绿色荧光蛋白标记的强毒鲁氏耶尔森菌，并在腹腔注射和浸泡攻毒后检测其在虹鳟鱼组织中的情况。
Dis Aquat Organ. 2005 Nov 28;67(3):267-72. doi: 10.3354/dao067267.
7
Type III flagellar protein export and flagellar assembly.III型鞭毛蛋白输出与鞭毛组装
Biochim Biophys Acta. 2004 Nov 11;1694(1-3):207-17. doi: 10.1016/j.bbamcr.2004.04.005.
8
A simple method for the detection of lipolytic activity of micro-organisms and some observations on the influence of the contact between cells and fatty substrates.一种检测微生物脂解活性的简单方法以及关于细胞与脂肪底物之间接触影响的一些观察结果。
Antonie Van Leeuwenhoek. 1957;23(1):15-22. doi: 10.1007/BF02545855.
9
Recovery of a new biogroup of Yersinia ruckeri from diseased rainbow trout (Oncorhynchus mykiss, Walbaum).从患病虹鳟鱼（Oncorhynchus mykiss，Walbaum）中分离出鲁氏耶尔森氏菌新生物群。
Syst Appl Microbiol. 2003 Mar;26(1):127-31. doi: 10.1078/072320203322337416.
10
Genetic analysis of pigment biosynthesis in Xanthobacter autotrophicus Py2 using a new, highly efficient transposon mutagenesis system that is functional in a wide variety of bacteria.利用一种新型的、高效的转座子诱变系统对自养黄色杆菌Py2中的色素生物合成进行遗传分析，该系统在多种细菌中均有功能。
Arch Microbiol. 2002 Sep;178(3):193-201. doi: 10.1007/s00203-002-0442-2. Epub 2002 Jun 18.
Zotero 插件