简短通讯：牛乳脂肪成分的全基因组扫描。II. 长链脂肪酸的数量性状基因座

Short communication: Genome-wide scan for bovine milk-fat composition. II. Quantitative trait loci for long-chain fatty acids.

作者信息

Schennink A, Stoop W M, Visker M H P W, van der Poel J J, Bovenhuis H, van Arendonk J A M

机构信息

Animal Breeding and Genomics Centre, Wageningen University, Wageningen, the Netherlands.

出版信息

J Dairy Sci. 2009 Sep;92(9):4676-82. doi: 10.3168/jds.2008-1965.

DOI:10.3168/jds.2008-1965

PMID:19700731

Abstract

We present the results of a genome-wide scan to identify quantitative trait loci (QTL) that contribute to genetic variation in long-chain milk fatty acids. Milk-fat composition phenotypes were available on 1,905 Dutch Holstein-Friesian cows. A total of 849 cows and their 7 sires were genotyped for 1,341 single nucleotide polymorphisms across all Bos taurus autosomes (BTA). We detected significant QTL on BTA14, BTA15, and BTA16: for C18:1 cis-9, C18:1 cis-12, C18:2 cis-9,12, CLA cis-9,trans-11, C18:3 cis-9,12,15, the C18 index, the total index, total saturated fatty acids, total unsaturated fatty acids (UFA), and the ratio of saturated fatty acids:unsaturated fatty acids on BTA14; for C18:1 trans fatty acids on BTA15; and for the C18 and CLA indices on BTA16. The QTL explained 3 to 19% of the phenotypic variance. Suggestive QTL were found on 16 other chromosomes. The diacylglycerol acyltransferase 1 (DGAT1) K232A polymorphism on BTA14, which is known to influence fatty acid composition, most likely explains the QTL that was detected on BTA14.

摘要

我们展示了一项全基因组扫描的结果，以确定影响长链乳脂肪酸遗传变异的数量性状位点（QTL）。有1905头荷兰荷斯坦-弗里生奶牛的乳脂肪组成表型数据。对总共849头奶牛及其7头公牛进行了基因分型，检测了牛（Bos taurus）所有常染色体（BTA）上的1341个单核苷酸多态性。我们在BTA14、BTA15和BTA16上检测到显著的QTL：在BTA14上，与C18:1顺-9、C18:1顺-12、C18:2顺-9,12、共轭亚油酸（CLA）顺-9,反-11、C18:3顺-9,12,15、C18指数、总指数、总饱和脂肪酸、总不饱和脂肪酸（UFA）以及饱和脂肪酸与不饱和脂肪酸的比例有关；在BTA15上，与C18:1反式脂肪酸有关；在BTA16上，与C18和CLA指数有关。这些QTL解释了3%至19%的表型变异。在其他16条染色体上发现了暗示性QTL。已知位于BTA14上的二酰甘油酰基转移酶1（DGAT1）K232A多态性会影响脂肪酸组成，很可能解释了在BTA14上检测到的QTL。