Huang Xin-Li, Zhou Xiao-Hong, Zhou Jun-Lin, Ding Chun-Hua, Xian Xiao-Hui
Department of Pathophysiology, Hebei Medical University, Shijiazhuang 050017, China.
Sheng Li Xue Bao. 2009 Aug 25;61(4):356-60.
The animal model of acute lung injury (ALI) caused by intravenous injection of lipopolysaccharides (LPS) and cultured human peripheral blood polymorphonuclear neutrophil (PMN) were used to study the effects of sodium hydrosulfide (NaHS), hydrogen sulfide (H2S) donor, on LPS-induced PMN accumulation, microvascular permeability and PMN apoptosis. Control group, NaHS group, LPS group and LPS + NaHS group were established both in in vivo and in vitro studies. Microvascular permeability, PMN accumulation in lung and apoptosis of PMN were detected. The results showed that: (1) In in vivo study, PMN accumulation in lung, the protein content in bronchoalveolar lavage fluid (BALF) and the Evans blue dye in lung tissue of LPS group were markedly higher than those of both sham operation group and LPS + NaHS group (P<0.05, P<0.01); (2) In in vitro study, the apoptotic rates of PMN in LPS group and NaHS group were significantly higher than that in control group (P<0.01), while compared with LPS group, LPS + NaHS group showed significantly higher apoptotic rate (P<0.01). These results suggest that NaHS attenuates LPS-induced microvascular permeability and alleviates ALI. PMN apoptosis induced by NaHS is possibly one of the potential mechanisms underlying the decrease of PMN accumulation in lung tissue.
采用静脉注射脂多糖（LPS）所致急性肺损伤（ALI）动物模型和培养的人外周血多形核中性粒细胞（PMN），研究硫化氢供体硫氢化钠（NaHS）对LPS诱导的PMN聚集、微血管通透性及PMN凋亡的影响。体内和体外研究均设立对照组、NaHS组、LPS组和LPS + NaHS组。检测微血管通透性、肺内PMN聚集及PMN凋亡情况。结果显示：（1）体内研究中，LPS组肺内PMN聚集、支气管肺泡灌洗液（BALF）中蛋白含量及肺组织伊文思蓝染料含量均显著高于假手术组和LPS + NaHS组（P<0.05，P<0.01）；（2）体外研究中，LPS组和NaHS组PMN凋亡率显著高于对照组（P<0.01），而与LPS组相比，LPS + NaHS组凋亡率显著更高（P<0.01）。这些结果表明，NaHS可减轻LPS诱导的微血管通透性并缓解ALI。NaHS诱导的PMN凋亡可能是肺组织中PMN聚集减少的潜在机制之一。
