通过微泡增强超声暴露用超顺磁性氧化铁对间充质干细胞进行体外标记：初步经验。
In vitro labeling of mesenchymal stem cells with superparamagnetic iron oxide by means of microbubble-enhanced US exposure: initial experience.
作者信息
Liu Zai-Yi, Wang Ying, Liang Chang-Hong, Li Xiao-Hong, Wang Guang-Yi, Liu Hong-Jun, Li Yan
机构信息
Department of Radiology, Guangdong General Hospital, 106 Zhong Shan Er Lu, Guangzhou, Guangdong Province 510080, People's Republic of China.
出版信息
Radiology. 2009 Oct;253(1):153-9. doi: 10.1148/radiol.2531081974. Epub 2009 Aug 25.
PURPOSE
To investigate the feasibility of magnetically labeling stem cells with superparamagnetic iron oxide (SPIO) by means of microbubble-enhanced ultrasonographic (US) exposure (MUE) and to study the effects of this approach--without secondary transfection agents--on the viability, proliferation activity, and differentiation capability of MUE-labeled stem cells.
MATERIALS AND METHODS
Institutional review board approval was obtained for this study. Human mesenchymal stem cells (MSCs) ([1 to 2] x 10(6)/mL) were studied in four experiment groups: sham exposure to US with microbubbles and SPIO (group A), exposure to US with SPIO but without microbubbles (group B), exposure to US with microbubbles and SPIO (group C), and sham exposure to US without SPIO or microbubbles (group D). Intracellular iron uptake was analyzed qualitatively at light and electron microscopy. The viability and proliferation activity of MSCs were evaluated. The adipogenic and osteogenic differentiation capability of the labeled MSCs was also evaluated. Ninety-five percent confidence intervals were derived for assessment of differences in cell viability and proliferation activity between groups C and D.
RESULTS
Light and electron microscopy revealed intracytoplasmic iron uptake and nearly 100% cell labeling efficiency. The MUE-labeled MSCs had unaltered viability and uncompromised proliferation activity compared with the nonlabeled MSCs. Similar to the nonlabeled MSCs, the MUE-labeled MSCs differentiated into adipogenic and osteogenic lineages.
CONCLUSION
Initial study results show that stem cells can be effectively labeled with SPIO by using MUE without secondary transfection agents and thus that MUE labeling is an appealing alternative cell-labeling approach that warrants investigation for intracellular magnetic labeling of stem cells.
SUPPLEMENTAL MATERIAL
http://radiology.rsna.org/lookup/suppl/doi:10.1148/radiol.2531081974/-/DC1.
目的
探讨通过微泡增强超声（US）暴露（MUE）用超顺磁性氧化铁（SPIO）对干细胞进行磁性标记的可行性，并研究这种方法（无需二次转染剂）对MUE标记干细胞的活力、增殖活性和分化能力的影响。
材料与方法
本研究获得机构审查委员会批准。在四个实验组中研究了人骨髓间充质干细胞（MSCs）（[1至2]×10⁶/mL）：假暴露于含微泡和SPIO的US（A组）、暴露于含SPIO但不含微泡的US（B组）、暴露于含微泡和SPIO的US（C组）以及假暴露于不含SPIO或微泡的US（D组）。在光学和电子显微镜下对细胞内铁摄取进行定性分析。评估MSCs的活力和增殖活性。还评估了标记的MSCs的成脂和成骨分化能力。得出95%置信区间以评估C组和D组之间细胞活力和增殖活性的差异。
结果
光学和电子显微镜显示胞质内铁摄取以及近100%的细胞标记效率。与未标记的MSCs相比，MUE标记的MSCs具有未改变的活力和未受损的增殖活性。与未标记的MSCs相似，MUE标记的MSCs分化为成脂和成骨谱系。
结论
初步研究结果表明，使用MUE无需二次转染剂即可用SPIO有效标记干细胞，因此MUE标记是一种有吸引力的替代细胞标记方法，值得对干细胞的细胞内磁性标记进行研究。
补充材料
http://radiology.rsna.org/lookup/suppl/doi:10.1148/radiol.2531081974/-/DC1
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