Laranjo Marta, Machado Jorge, Young J Peter W, Oliveira Solange
Departamento de Biologia, Universidade de Evora, Portugal.
FEMS Microbiol Ecol. 2004 Apr 1;48(1):101-7. doi: 10.1016/j.femsec.2003.12.015.
Chickpea rhizobia isolated from Portuguese soils were assigned to the genus Mesorhizobium by 16S-rDNA sequencing. High species diversity was found within populations of an agricultural region in the south of Portugal. Besides the expected Mesorhizobium ciceri and M. mediterraneum, some isolates were close to M. loti or M. tianshanense and some formed a clade that may represent a new species. A new PCR-based approach, named direct amplified polymorphic DNA (DAPD) analysis, supported the 16S-based phylogeny. This suggests that this method could be used as a molecular tool to assess genetic relationships. Evaluation of genetic diversity by 16S-rDNA sequence, DAPD and protein profiles showed different levels of heterogeneity in natural populations.
通过16S - rDNA测序，从葡萄牙土壤中分离出的鹰嘴豆根瘤菌被归类为中慢生根瘤菌属。在葡萄牙南部一个农业区域的种群中发现了高度的物种多样性。除了预期的鹰嘴豆中慢生根瘤菌和地中海中慢生根瘤菌外，一些分离株与百脉根中慢生根瘤菌或天山中慢生根瘤菌相近，还有一些形成了一个可能代表新物种的进化枝。一种名为直接扩增多态性DNA（DAPD）分析的基于PCR的新方法支持了基于16S的系统发育。这表明该方法可作为评估遗传关系的分子工具。通过16S - rDNA序列、DAPD和蛋白质谱对遗传多样性的评估显示，自然种群中存在不同程度的异质性。
