Ferrer Elizabeth, Martínez-Escribano José Angel, Barderas María Eugenia González, González Luis Miguel, Cortéz María Milagros, Dávila Iris, Harrison Leslie J S, Parkhouse R Michael E, Gárate Teresa
Instituto de Salud Carlos III, Centro Nacional de Microbiología, 28220 Majadahonda, Madrid, Spain.
Mol Biochem Parasitol. 2009 Dec;168(2):168-71. doi: 10.1016/j.molbiopara.2009.08.003. Epub 2009 Aug 25.
Ts8B2 is a gene which encodes for a member of the Taenia solium metacestode 8kDa antigen family. Since the Ts8B2-GST recombinant protein compares very favourably with other diagnostic antigens, and in order to study the antigenic nature and structure of this molecule, the Ts8B2 was expressed in prokaryotic and eukaryotic systems. The diagnostic potential of the recombinant Ts8B2 proteins was evaluated by enzyme-linked immunosorbent assays (ELISA) using a collection of serum and cerebrospinal fluid (CSF) samples from patients with clinically defined neurocysticercosis (NCC), and also sera from T. solium infected pigs. Despite the predicted glycosylation of the Ts8B2-Bac recombinant protein, there was very little difference in assay sensitivity/specificity when the Ts8B2 reagent was expressed in either prokaryotic or eukaryotic systems, suggesting that peptidic Ts8B2 epitopes are immunodominant in porcine cysticercosis and human neurocysticercosis. Conveniently, production of recombinant Ts8B2 in Escherichia coli is economical and facile, making it a feasible and practical choice as a diagnostic reagent for use in endemic areas. The Ts8B2 ELISA is particularly useful for the diagnosis of active as opposed to inactive cases of NCC and conduct of the assay is also facilitated by the fact that assay sensitivity is significantly greater when serum as opposed to CSF samples are employed.
Ts8B2是一个编码猪带绦虫囊尾蚴8 kDa抗原家族成员的基因。由于Ts8B2 - GST重组蛋白与其他诊断抗原相比具有很大优势，并且为了研究该分子的抗原性质和结构，Ts8B2在原核和真核系统中进行了表达。使用来自临床确诊的神经囊尾蚴病（NCC）患者的血清和脑脊液（CSF）样本以及猪带绦虫感染猪的血清，通过酶联免疫吸附测定（ELISA）评估重组Ts8B2蛋白的诊断潜力。尽管预测Ts8B2 - Bac重组蛋白存在糖基化，但当Ts8B2试剂在原核或真核系统中表达时，测定的敏感性/特异性差异很小，这表明肽类Ts8B2表位在猪囊尾蚴病和人类神经囊尾蚴病中具有免疫优势。方便的是，在大肠杆菌中生产重组Ts8B2既经济又简便，使其成为在流行地区用作诊断试剂的可行且实用的选择。Ts8B2 ELISA对于诊断活跃型而非非活跃型NCC病例特别有用，并且当使用血清而非CSF样本时测定敏感性显著更高这一事实也便于进行该测定。
