Ferrer Elizabeth, Cortéz M Milagros, Cabrera Zully, Rojas Glenda, Dávila Iris, Alarcón de Noya Belkis, Pérez Hilda A, Fernandez Isis, Romero Haidee Urdaneta, Harrison Leslie J S, Parkhouse R Michael E, Gárate Teresa
Centro de Investigaciones Biomédicas, Universidad de Carabobo, Maracay, Venezuela.
Trans R Soc Trop Med Hyg. 2005 Aug;99(8):568-76. doi: 10.1016/j.trstmh.2005.02.003.
This study evaluates five synthetic peptides derived from four, potentially protective, Taenia saginata oncosphere molecules for the serodiagnosis of T. solium cysticercosis/neurocysticercosis in three distinct Venezuelan endemic regions. The peptides, all of which have been described previously, are designated HP6-3, Ts45W-1, Ts45W-5, Ts45S-10 and TEG-1. In clinically verified and seropositive hospital cases, combining the results of three of the individual peptide-based ELISAs (HP6-3, Ts45W-1 and Ts45W-5) afforded the best balance between sensitivity (85%) and specificity (83.5%), a significant improvement on the 63.6% specificity obtained with the routinely employed T. solium cyst-fluid-based ELISA. Similarly, in the seropositive Venezuelan endemic zone samples, 89.09% of Amerindians, 77.27% of symptomatic rural subjects and 67.83% of non-symptomatic rural subjects were also classed as seropositive by the combined peptide-based ELISAs. The profile of antibody recognition to individual peptides varied between the different groups of samples examined. The relevance of the above findings for the serology and prognosis of T. solium cysticercosis/neurocysticercosis in hospital- and field-based situations is discussed.
本研究评估了源自四种可能具有保护作用的牛带绦虫六钩蚴分子的五种合成肽，用于在委内瑞拉三个不同的地方性流行区域对猪带绦虫囊尾蚴病/神经囊尾蚴病进行血清学诊断。这些肽均已在之前被描述过，分别命名为HP6 - 3、Ts45W - 1、Ts45W - 5、Ts45S - 10和TEG - 1。在经临床验证且血清学呈阳性的医院病例中，将三种基于单个肽的酶联免疫吸附测定（ELISA）（HP6 - 3、Ts45W - 1和Ts45W - 5）的结果相结合，在敏感性（85%）和特异性（83.5%）之间实现了最佳平衡，相较于常规使用的基于猪带绦虫囊液的ELISA所获得的63.6%的特异性有了显著提高。同样，在血清学呈阳性的委内瑞拉地方性流行区域样本中，基于肽的联合ELISA也将89.09%的美洲印第安人、77.27%有症状的农村受试者和67.83%无症状的农村受试者归类为血清学阳性。在所检测的不同样本组中，对各个肽的抗体识别情况各不相同。本文讨论了上述研究结果对于医院和现场环境下猪带绦虫囊尾蚴病/神经囊尾蚴病血清学及预后的相关性。
