Azadmehr Abbas, Pourfathollah Ali Akbar, Amirghofran Zahra, Hassan Zuhair Mohammad, Moazzeni Seyed Mohammad
Department of Immunology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran.
Cell Immunol. 2009;260(1):28-32. doi: 10.1016/j.cellimm.2009.07.003. Epub 2009 Aug 3.
The discovery of dendritic cells (DCs) as professional antigen presenting cells has opened up new possibilities for their use in the development of tumor vaccines. We investigated the effect of the CD8alpha(+) DCs loaded with heat-treated tumor lysate (HTL) as a vaccine in tumor immunotherapy. The HTL loaded CD8alpha(+) DCs, TL loaded CD8alpha(+) DCs and unloaded CD8alpha(+) DCs were subcutaneously injected in the fibrosarcoma-bearing mice. The splenocyte proliferation and the shifting of Th1/Th2 response were measured. The results indicated a significant increase in the lymphocytes proliferation and the IFN-gamma production in the test group of mouse splenocytes. According to the results, HTL loaded CD8alpha(+) DCs vaccine significantly decreased tumor growth and longer survival than the other immunized animals. These findings show that anti-tumor immune response against the fibrosarcoma can be induced by HTL loaded CD8alpha(+) DCs and may provide a useful therapeutic model for development of approaches to tumor treatments.
树突状细胞（DCs）作为专职抗原呈递细胞的发现为其在肿瘤疫苗开发中的应用开辟了新的可能性。我们研究了负载热处理肿瘤裂解物（HTL）的CD8α(+) DCs作为疫苗在肿瘤免疫治疗中的效果。将负载HTL的CD8α(+) DCs、负载肿瘤裂解物（TL）的CD8α(+) DCs和未负载的CD8α(+) DCs皮下注射到荷纤维肉瘤小鼠体内。检测脾细胞增殖以及Th1/Th2反应的转变。结果表明，小鼠脾细胞实验组中淋巴细胞增殖和干扰素-γ产生显著增加。根据结果，负载HTL的CD8α(+) DCs疫苗比其他免疫动物显著降低了肿瘤生长并延长了生存期。这些发现表明，负载HTL的CD8α(+) DCs可诱导针对纤维肉瘤的抗肿瘤免疫反应，并可能为开发肿瘤治疗方法提供有用的治疗模型。
