Hooper I R, Rooth M, Sambles J R
School of Physics, University of Exeter, Stocker Road, Exeter, Devon, EX4 4QL, UK.
Biosens Bioelectron. 2009 Oct 15;25(2):411-7. doi: 10.1016/j.bios.2009.07.023. Epub 2009 Aug 3.
This work demonstrates a dual-channel surface plasmon sensing device based upon the differential surface plasmon ellipsometry method. Experiments showing the removal of common-mode noise, the linearity of response to changes in refractive index and the use of a reference sensing channel in a comparative protein - antibody study are presented. The ease of multiplexing inherent within the differential surface plasmon ellipsometry method allows for the potential development of sensing systems with many parallel channels. Coupled with the use of reference channels for common-mode noise subtraction, multi-analyte sensing at detection limits comparable to those of the best single channel surface plasmon sensors currently available becomes a realistic goal. In this paper we present the first multi-channel sensing results obtained using the differential surface plasmon ellipsometry method and provide a comparison between the linearity of response to changes in refractive index and a protein-antibody binding study with a commercially available SPR instrument (Nomadics SensiQ Discovery).
这项工作展示了一种基于差分表面等离子体椭圆偏振法的双通道表面等离子体传感装置。文中呈现了相关实验，这些实验展示了共模噪声的消除、对折射率变化响应的线性以及在蛋白质 - 抗体对比研究中参考传感通道的使用。差分表面等离子体椭圆偏振法固有的多路复用简易性使得具有多个并行通道的传感系统有潜在的发展可能。再加上使用参考通道来减去共模噪声，在检测限方面实现多分析物传感，使其与目前可用的最佳单通道表面等离子体传感器相当，这成为了一个现实的目标。在本文中，我们展示了使用差分表面等离子体椭圆偏振法获得的首个多通道传感结果，并将折射率变化响应的线性与使用市售表面等离子体共振仪器（Nomadics SensiQ Discovery）进行的蛋白质 - 抗体结合研究作了比较。
