Bae Young Min, Oh Byung-Keun, Lee Woochang, Lee Won Hong, Choi Jeong-Woo
Department of Chemical and Biomolecular Engineering, Sogang University, 1 Sinsu-Dong, Mapo-Gu, Seoul 121-742, South Korea.
Biosens Bioelectron. 2004 Nov 1;20(4):895-902. doi: 10.1016/j.bios.2004.03.032.
Imaging ellipsometry (IE) was used to detect the binding of insulin to its antibody on a solid surface. The modification of a gold surface with 11-mecaptoundecanoic acid (11-MUA), the adsorption of protein G, and antibody immobilization onto the protein G layer were confirmed by surface plasmon resonance. Ellipsometric images and ellipsometric angles of the surface antibody were acquired using the IE system by off-null ellipsometry. Ellipsometric images of antigen binding to the antibody were acquired, and their mean optical intensities estimated. Changes in mean optical intensity indicated that the detection range for insulin was from 10 ng/ml to 100 microg/ml.
成像椭偏仪（IE）用于检测胰岛素与其在固体表面抗体的结合。通过表面等离子体共振确认了用11-巯基十一烷酸（11-MUA）修饰金表面、蛋白G的吸附以及抗体固定到蛋白G层上。使用IE系统通过偏零椭偏法获取表面抗体的椭偏图像和椭偏角。获取抗原与抗体结合的椭偏图像，并估计其平均光强度。平均光强度的变化表明胰岛素的检测范围为10 ng/ml至100 μg/ml。
