Ntinas Achilleas, Iliadis Stavros, Alvanou Achparaki Athanasia, Vrochides Dionisios, Pitoulias Georgios, Papageorgiou Georgios, Spyridis Charalambos, Papadimitriou Dimitrios, Karamanos Dimitrios, Gerasimidis Thomas
5th Surgery, Hippokration Hospital, Aristotle University of Thessaloniki, Thessaloniki, Greece.
Vasc Endovascular Surg. 2010 Feb;44(2):81-8. doi: 10.1177/1538574409344437. Epub 2009 Aug 27.
To evaluate the effect of intraluminal administration of oxygenated perfluorocarbons (PFCs) on small intestine's viability in an experimental model of acute ischemia-reperfusion (I/R).
Twenty rabbits were divided in four groups: sham-operated controls (group A), acute I/R (group B), acute I/R plus infusion of PFCs 30 min before ischemia (group C), and acute I/R plus infusion of PFCs 30 min before reperfusion (group D). Malondialdehyde (MDA) tissue levels and d-lactate blood samples were taken. All tissue sections were examined under light microscope.
Mean MDA levels in group A: 1.79 +/- 0.97 at 0 min, 2.25 +/- 1.76 at 120 min and 3.70 +/- 1.76 nmols/g at 180 min. Group B: 2.60 +/- 0.58 at 0 min, 4.20 +/- 0.58 at 120 min and 5.48 +/- 2.01 at 180 min. Group C: 1.54 +/- 0.85 at 0 min, 1.14 +/- 0.37 at 120 min and 0.59 +/- 0.35 at 180 min. Group D: 2.12 +/- 0.62 at 0 min, 3.97 +/- 0.70 at 120 min and 2.32 +/- 0.37 at 180 min (p < 0.05). Mean d-lactate levels in group A: at 0 min 36.45 +/- 1.99, at 120 min 39.10 +/- 2.37 and at 180 min 40.05 +/- 2.13 mg/dl. Group B: 61.23 +/- 11.03 at 0 min, 74.84 +/- 10.70 at 120 min and 89.90 +/- 9.29 at 180 min. Group C: at 0 min 51.05 +/- 10.36, at 120 min 56.07 +/- 11.27 and at 180 min 57.20 +/- 11.19. Group D: 64.36 +/- 5.26 at 0 min, 72.55 +/- 7.19 at 120 min and 77.02 +/- 9.41 at 180 min (p < 0.05). Histopathological analysis indicated a significant improvement in the groups of oxygenated PFCs compared with I/R group.
Intraluminal administration of oxygenated PFCs seems that protect the intestine from the I/R injury.
在急性缺血再灌注（I/R）实验模型中评估腔内给予氧化全氟碳化合物（PFCs）对小肠活力的影响。
将20只兔子分为四组：假手术对照组（A组）、急性I/R组（B组）、缺血前30分钟急性I/R加输注PFCs组（C组）、再灌注前30分钟急性I/R加输注PFCs组（D组）。采集丙二醛（MDA）组织水平和d-乳酸血样。所有组织切片在光学显微镜下检查。
A组MDA平均水平：0分钟时为1.79±0.97，1
