Oxygenated perfluorocarbons protect the intestine from the ischemia/reperfusion injury in rabbits.
作者信息
Ntinas Achilleas, Vrochides Dionisios, Iliadis Stavros, Papageorgiou Georgios, Alvanou-Achparaki Athanasia, Papadimitriou Dimitrios, Spiridis Charalambos, Gerasimidis Thomas
机构信息
5th Department of Surgery, Hippokration Hospital, Aristotle University of Thessaloniki, Thessaloniki, Greece.
出版信息
Vasc Endovascular Surg. 2011 Jul;45(5):426-32. doi: 10.1177/1538574411402223. Epub 2011 May 13.
OBJECTIVE
To investigate whether intraluminal administration of oxygenated perfluorocarbons (PFCs) protects the enterocyte from acute ischemia-reperfusion (I/R) injury.
MATERIALS AND METHODS
Twenty rabbits were divided in 4 groups: sham-operated controls (group A), acute I/R (group B), acute I/R plus infusion of oxygenated PFCs 30 minutes before ischemia (group C), and acute I/R plus infusion of oxygenated PFCs 30 minutes before reperfusion (group D). Serum creatine phosphokinase (CPK) and mucosal disaccharidase activity were examined. Intestinal biopsies were obtained for electron microscopy study.
RESULTS
Group B CPK mean values are 3495.2 ± 157.35 and 4855 ± 350.21 U/L. Group C: 2674.6 ± 265.87 and 3231 ± 232.30. Group D: 2382.2 ± 102.90 and 3217.6 ± 185.61 at 120 and 180 minutes (P<.05). At 180 minutes, maltase and sucrose values were 33.63, 51.88, 8.45, and 19.91, and 17.99, 22.87, 6.62, and 14.24 µmol/min per g for groups A, B, C, and D, respectively (P<.05). Histopathology showed the least cellular deterioration in PFC groups.
CONCLUSION
Oxygenated PFCs protect the enterocyte during bowel I/R.
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