Koshelev Irina, Huang Rong, Graber Timothy, Meron Mati, Muir J Lewis, Lavender William, Battaile Kevin, Mulichak Anne M, Keefe Lisa J
IMCA-CAT, The Center for Advanced Radiation Sources, University of Chicago, IL 60637, USA.
J Synchrotron Radiat. 2009 Sep;16(Pt 5):647-57. doi: 10.1107/S0909049509022353. Epub 2009 Jul 9.
The IMCA-CAT bending-magnet beamline was upgraded with a collimating mirror in order to achieve the energy resolution required to conduct high-quality multi- and single-wavelength anomalous diffraction (MAD/SAD) experiments without sacrificing beamline flux throughput. Following the upgrade, the bending-magnet beamline achieves a flux of 8 x 10(11) photons s(-1) at 1 A wavelength, at a beamline aperture of 1.5 mrad (horizontal) x 86 microrad (vertical), with energy resolution (limited mostly by the intrinsic resolution of the monochromator optics) deltaE/E = 1.5 x 10(-4) (at 10 kV). The beamline operates in a dynamic range of 7.5-17.5 keV and delivers to the sample focused beam of size (FWHM) 240 microm (horizontally) x 160 microm (vertically). The performance of the 17-BM beamline optics and its deviation from ideally shaped optics is evaluated in the context of the requirements imposed by the needs of protein crystallography experiments. An assessment of flux losses is given in relation to the (geometric) properties of major beamline components.
IMCA-CAT弯铁光束线配备了准直镜进行升级,以便在不牺牲光束线通量通过率的情况下,实现进行高质量多波长和单波长反常衍射(MAD/SAD)实验所需的能量分辨率。升级后,弯铁光束线在波长为1 Å时,在光束线孔径为1.5 mrad(水平)×86 μrad(垂直)的条件下,通量达到8×10¹¹ 光子·秒⁻¹,能量分辨率(主要受单色仪光学元件的固有分辨率限制)ΔE/E = 1.5×10⁻⁴(在10 kV时)。该光束线在7.5 - 17.5 keV的动态范围内运行,并向样品提供尺寸(半高宽)为240 μm(水平)×160 μm(垂直)的聚焦光束。根据蛋白质晶体学实验的需求所提出的要求,评估了17-BM光束线光学元件的性能及其与理想形状光学元件的偏差。针对主要光束线部件的(几何)特性,给出了通量损失的评估结果。