Gandhi Santosh Vilashchand, Khan Samina I, Jadhav Rashmi T, Jadhav Saket S, Jadhav Gaurav A
AISSMS College of Pharmacy, Department of Pharmaceutical Analysis, Pune, India.
J AOAC Int. 2009 Jul-Aug;92(4):1064-7.
A new, simple, high-performance thin-layer chromatographic method for determination of rabeprazole sodium (RAB) and domperidone (DOM) in combined tablet dosage form has been developed and validated. The mobile phase was toluene-acetone-methanol (4.5 + 4.5 + 0.5, v/v/v) with UV detection at 285 nm. The retention factors for RAB and DOM were found to be 0.53 +/- 0.12 and 0.32 +/- 0.20. The method was validated with respect to linearity, accuracy, precision, and robustness. Beer's law was obeyed in the concentration range of 50-800 ng/band for both RAB and DOM. The method has been successfully applied for the analysis of drugs in a pharmaceutical formulation.
已开发并验证了一种用于测定复方片剂剂型中雷贝拉唑钠（RAB）和多潘立酮（DOM）的新型、简单、高效的薄层色谱方法。流动相为甲苯 - 丙酮 - 甲醇（4.5 + 4.5 + 0.5，v/v/v），在285 nm处进行紫外检测。RAB和DOM的保留因子分别为0.53±0.12和0.32±0.20。该方法在线性、准确度、精密度和稳健性方面得到了验证。RAB和DOM在50 - 800 ng/条带的浓度范围内均符合比尔定律。该方法已成功应用于药物制剂中药物的分析。
