Hubicka Urszula, Krzek Jan, Woltyńska Hanna, Stachacz Bozena
Collegium Medicum of Jagiellonian University, Department of Inorganic and Analytical Chemistry, Medyczna 9, 30-688 Kraków, Poland.
J AOAC Int. 2009 Jul-Aug;92(4):1068-75.
A TLC-densitometric method has been developed for simultaneous identification and quantitative determination of amikacin, gentamicin, kanamycin, neomycin, netilmicin, and tobramycin. This separation of antibiotics was achieved on silica gel TLC plates without a fluorescent indicator and with methanol-25% ammonia-chloroform (3 + 2 + 1, v/v/v) as the mobile phase. The densitometric measurements were made at 500 nm after detection with a 0.2% ninhydrin solution in ethanol. Under these conditions, good separation of the chosen aminoglycosides was obtained. The method is distinguished by high sensitivity, with the LOD from 0.25 microg for amikacin to 1.00 microg for gentamicin and the LOQ from 0.5 microg for amikacin to 1.65 microg for gentamicin, and a wide linearity range 0.75-6.25 microg/spot for amikacin and netilmicin and 1.5-12.50 microg/spot for other antibiotics. The precision of the determination was very good; RSD varied in the range 0.3-0.6%.
已开发出一种薄层色谱-密度测定法，用于同时鉴别和定量测定阿米卡星、庆大霉素、卡那霉素、新霉素、奈替米星和妥布霉素。这些抗生素在不含荧光指示剂的硅胶薄层色谱板上进行分离，流动相为甲醇-25%氨水-氯仿（3 + 2 + 1，v/v/v）。用0.2%茚三酮乙醇溶液检测后，在500 nm处进行密度测定。在这些条件下，所选氨基糖苷类药物得到了良好的分离。该方法具有高灵敏度，阿米卡星的检测限为0.25 μg，庆大霉素为1.00 μg，定量限阿米卡星为0.5 μg，庆大霉素为1.65 μg，线性范围宽，阿米卡星和奈替米星为0.75 - 6.25 μg/斑点，其他抗生素为1.5 - 12.50 μg/斑点。测定的精密度非常好；相对标准偏差在0.3 - 0.6%范围内。
