Hu Yu-Fei, Li Gong-Ke, Zhang Zhu-Jun
School of Chemistry and Chemical Engineering, Sun Yat-sen University, 135 Xingang Xi, Guangzhou 510275, China.
Department of Chemistry, Institute of Analytical Science, Southwest University, Beibei, Chongqing 400715, China.
J Pharm Anal. 2013 Oct;3(5):360-366. doi: 10.1016/j.jpha.2012.12.011. Epub 2013 Jan 11.
A novel chemiluminescence (CL) reaction was based on the oxidizing reaction of luminol by the trivalent copper-periodate complex (K[Cu(HIO)], DPC) in alkaline medium. The CL intensity could be enhanced in the presence of amikacin sulfate (AKS). A new CL method was developed for the determination of AKS by coupling with flow injection (FI) technology. Because of the distinctive oxidative effect of DPC, the luminol-based CL reaction could occur at a low concentration of 10 M. The relative CL intensity was proportional to the concentration of AKS in the range of 4.0×10-4.0×10 g/mL with the detection limit of 1.2×10 g/mL. The relative standard deviation was 2.1% for 8.0×10 g/mL AKS (=9). The proposed method was successfully applied to the direct determination of AKS at the level of ng/mL in serum samples. The recovery varied from 97.0% to 106.3%. A possible mechanism of the CL reaction was discussed in detail by relating to the CL kinetic characteristics and electrochemical activities of the oxidant DPC.
一种新型化学发光（CL）反应基于鲁米诺在碱性介质中被三价铜 - 高碘酸盐配合物（K[Cu(HIO)]，DPC）氧化的反应。在硫酸阿米卡星（AKS）存在下，化学发光强度会增强。结合流动注射（FI）技术开发了一种测定AKS的新化学发光方法。由于DPC独特的氧化作用，基于鲁米诺的化学发光反应可在低至10⁻⁶ M的浓度下发生。相对化学发光强度与AKS浓度在4.0×10⁻⁷ - 4.0×10⁻⁵ g/mL范围内成正比，检测限为1.2×10⁻⁷ g/mL。对于8.0×10⁻⁶ g/mL的AKS（n = 9），相对标准偏差为2.1%。所提出的方法成功应用于血清样品中纳克/毫升水平AKS的直接测定。回收率在97.0%至106.3%之间。通过关联氧化剂DPC的化学发光动力学特征和电化学活性，详细讨论了化学发光反应的可能机制。
