Huang Guo-Yong, Wang You-Shao
Key Laboratory of Tropical Marine Environmental Dynamics, South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou 510301, China.
Chemosphere. 2009 Nov;77(7):1026-9. doi: 10.1016/j.chemosphere.2009.07.073. Epub 2009 Aug 27.
In this paper, we aimed to assess the roles of metallothioneins (MTs) in heavy metal tolerance by analyzing the expression level of BgMT2 in leaves of Bruguiera gymnorrhiza in response to heavy metals. Eight-month-old B. gymnorrhiza seedlings were exposed to different concentrations of zinc (Zn), copper (Cu) or lead (Pb) for 1, 3 and 7 d. A Real-time quantitative PCR protocol was developed to directly evaluate the expression of BgMT2, using 18S rRNA as a reference gene. Real-time quantitative PCR analysis demonstrated BgMT2 mRNA expression was regulated by Zn, Cu and Pb, but the regulation pattern was different for the three metals tested. Significant increase in the transcript level of BgMT2 was also found in response to Zn, Cu and Pb in some experimental conditions. Our results confirm that BgMT2 gene is involved in the regulation of Zn, Cu and Pb in B. gymnorrhiza leaves.
在本文中，我们旨在通过分析白骨壤叶片中BgMT2的表达水平对重金属的响应，来评估金属硫蛋白（MTs）在重金属耐受性中的作用。将八个月大的白骨壤幼苗暴露于不同浓度的锌（Zn）、铜（Cu）或铅（Pb）中1、3和7天。开发了一种实时定量PCR方案，以18S rRNA作为参考基因，直接评估BgMT2的表达。实时定量PCR分析表明，BgMT2 mRNA表达受Zn、Cu和Pb的调节，但三种测试金属的调节模式不同。在某些实验条件下，还发现BgMT2转录水平因Zn、Cu和Pb而显著增加。我们的结果证实，BgMT2基因参与了白骨壤叶片中Zn、Cu和Pb的调节。
