Université de Limoges, GRESE EA 4330, 123 avenue Albert Thomas 87060, Limoges, France.
Aquat Toxicol. 2009 Oct 4;94(4):255-64. doi: 10.1016/j.aquatox.2009.07.007. Epub 2009 Jul 17.
Copper sulphate is a common algaecide applied to ponds in order to keep phytoplanktonic blooms under control, especially those prone to cyanobacterial development. The copper toxicity was evaluated for two phytoplanktonic species with the same size and shape: one cyanobacteria Microcystis aeruginosa and one chlorophyceae Chlorella vulgaris. Under controlled conditions in order to keep free copper concentration constant, three bioassays were carried out: growth inhibition tests and two sets of flow cytometric analysis (autofluorescence and esterase activity inhibition). The results showed: (1) that regardless of the cell type, as copper concentrations increased, cell division rate and autofluorescence decreased, (2) a difference in sensitivity according to the length of exposure to copper: 24 or 48 h, and (3) that M. aeruginosa showed a greater sensitivity than C. vulgaris in the 24h copper exposure assay according to esterase activity data.
硫酸铜是一种常见的杀藻剂，用于池塘中以控制浮游植物水华，特别是那些容易产生蓝藻的水华。本研究评估了铜对两种大小和形状相同的浮游植物的毒性：一种蓝藻铜绿微囊藻和一种绿藻小球藻。在控制条件下，为了保持自由铜浓度恒定，进行了三种生物测定：生长抑制试验和两组流式细胞术分析（自发荧光和酯酶活性抑制）。结果表明：（1）无论细胞类型如何，随着铜浓度的增加，细胞分裂率和自发荧光降低；（2）根据暴露于铜的时间长短存在敏感性差异：24 或 48 小时；（3）根据酯酶活性数据，在 24 小时铜暴露试验中，铜绿微囊藻比小球藻表现出更高的敏感性。
